香芹酚通过降低一氧化氮水平防治大鼠脑缺血/再灌注损伤的研究

目的研究香芹酚通过降低一氧化氮水平对大鼠脑缺血/再灌注(CI/R)损伤的保护作用。方法制做大鼠脑中动脉闭塞(MCAO)2 h后再灌注24 h模型,将大鼠随机分为5组:假手术组,模型组,香芹酚低、中、高剂量组。实验终末,评价神经功能及脑梗死面积,并检测脑组织中NO的含量。结果与模型组相比,香芹酚组神经功能学评分显著下降(P<0.05),大鼠脑梗死面积亦显著减少(P<0.05)。模型组中再灌注24 h后,大鼠缺血侧和自身对照侧皮质及海马NO的含量异常升高(P<0.05);香芹酚组缺血侧和自身对照侧皮质及海马NO的含量明显降低(P<0.05)。结论香芹酚对大鼠CI/R损伤有保护作用,其机制与降低CI/R损伤时的脑组织中的NO水平可能相关。

Carvacrol against cerebral ischemia/reperfusion injury in rats by lowing the content of NO

Objective To determine the protection of carvacrol against cerebral ischemia/reperfusion（CI/R） injury in rats by lowing the content of NO. Methods The rats were subjected to CI/R by a thread for middle cerebral artery occlusion（MCAO）. After 2 h of cerebral ischemia, the middle cerebral artery was reperfused for 24 h. The rats were randomly divided into 5 groups： a sham group, a model group, and a carvacrol low, middle and high dose group. The neurologic function scores and infarct zone were estimated. The content of NO in the cerebral tissue was also measured by nitrite reduction. Results Compared with the model group, the neurologic function scores and infarct zone in the carvacrol group were significantly decreased（P 0.05）. After 24 h CI/R, the content of NO in the cerebral cortex and the hippocampal region of the ischemia and self-control side of model group was significantly more than that of the sham group（P 0.05）. The content of NO in the cerebral cortex and the hippocampal region of the ischemia and self-control side of the carvacrol group was significantly less than that of the model group（P 0.05）. Conclusion Carvacrol has excellent protective effect against CI/R injury. The mechanism may be related to the content of NO in the cerebral tissue.