嗅粘膜移植联合应用大剂量甲基强的松龙修复急性脊髓损伤

目的探讨嗅粘膜髓内移植联合应用大剂量甲基强的松龙修复脊髓损伤的可行性和疗效,观察甲基强的松龙与嗅鞘细胞的协同作用。方法将Wistar大鼠做成脊髓半切模型,随机分成髓内单独移植嗅粘膜组(OM)、嗅粘膜联合应用甲基强的松龙组(OM MP)、单独应用甲基强的松龙组(MP)和对照组(CG)共四组,分期进行联合行为学综合评分(CBS评分)及组织学检查,评价各组脊髓修复情况。结果CBS评分OM组及OM MP组较MP组及CG组在3周后各时段差别有显著意义(P<0.01);OM组和OM MP组没有明显差别(P>0.05);MP组与CG组有显著差别(P<0.05)。OM组及OM MP组组织学改变与MP组及CG组有显著差别,前两组间无显著差别。结论嗅粘膜髓内移植联合应用大剂量甲基强的松龙修复脊髓损伤具有很好的疗效,但未见MP与OM的明显协同作用。

Study on Repair of adult rat spinal cord injury by transplants of olfactory mucosa and administration of a high dose of Methylprednisolone

Objective: To investigate the synergetic effect and possibility of spinal cord injury repaired by transplantation of olfactory mucosa and methylprednisolone in adult rat.Methods: Wistar rats with T10 spinal cord hemisection were divided into 4 groups: olfactory mucosa group(OM),olfactory mucosa in combination with mthylprednisolone group(OM MP),methylprednisolone group(MP) and control group(CG) respectively.The functional recovery of spinal cord injury was observed with combined behavioral score(CBS) and pathological anatomy at different phases.The tissue sections were made at 5 weeks and 10 weeks postoperatively to observe the axon regeneration and the survival of olfactory ensheathing cells.Results: The CBS showed that rats treated with OM and OM MP had more improvement than that of MP and control group at series of phases after operation 3 weeks(p<0.01),but had not significant difference between OM group and OM MP group(p>0.05).The pathological section showed same results as CBS.Conclusion: The method of treating spinal cord injury by transplanted transplants of olfactory mucosa and administration of a high dose of Methylprednisolone had good curative effect,but had not significant synergetic effect.