胶质瘤中miR-383与其靶向circ_001634相互调控机制及作用研究

目的研究脑胶质瘤中circ_001634的表达以及与miR-383的相互调控关系及在胶质瘤发生发展中的作用。方法实时定量聚合酶链反应(Real-time PCR)检测脑胶质瘤组织中circ_001634的表达情况,及分别过表达circ_001634与miR-383后对相互的影响,非锚定依赖性生长实验检测转染circ_001634后胶质瘤U251细胞生长情况,Western blot检测miR-383下游基因CCND1蛋白表达情况。结果 circ_001634在胶质瘤中的表达显著升高;过表达circ_001634能抑制胶质瘤细胞生长,且能下调miR-383表达,并抑制CCND1表达;同时过表达miR-383,也能够抑制circ_001634表达。结论胶质瘤特异性circ_001634表达升高是miR-383表达下调和功能受抑制重要调控机制,circ_001634与miR-383的相互调控很可能是胶质瘤发生发展的重要原因。

The mechanism and function of the mutual regulation between miR-383 and its targeted circ 001634 in glioma

Objective To investigate the relationship and function of the mutual regulation be-tween miR-383 and circ 001634 which expressd in glioma. Methods Real-time PCR was applied to de-termine the expression of circ 001634 in glioma tissues,and explore the effects on each other when miR-383 and circ 001634 were overexpressed respectively. Anchorage-independent growth assay was used to determine the growth of U251cells after transfected with circ 001634. Western blot was applied to deter-mine the expression of CCND1,the downstream gene of miR-383. Results The expression of circ 001634 was upregulated in glioma tissues. Overexpression of circ 001634 could inhibit the growth of glioma cells, and downregulate the expression of miR-383 and it′s downstream gene CCND1. Meanwhile,overexpression of miR-383 could also inhibit the expression of circ 001634. Conclusion The upregulation of glioma specific circ 001634 is an important mechanism related to the downregulation of miR-383 and it′s function inhibiton. The mutual regulation between miR-383 and circ 001634 might be an important cause of the genesis and development of glioma.