尼美舒利对食管鳞癌细胞的增生抑制和诱导凋亡作用

目的研究特异性环氧化酶-2抑制剂尼美舒利对食管鳞癌细胞的增生抑制和诱导凋亡作用。方法EC109细胞与不同浓度的尼美舒利共同孵育。1)用MTT方法研究细胞增生抑制作用;2)用荧光标记的流式细胞检测技术研究尼美舒利的诱导凋亡作用;3)检测细胞培养上清液中前列腺素E2质量浓度,观察尼美舒利对环氧化酶功能的影响。结果尼美舒利抑制食管鳞癌细胞增生,呈浓度及时间依赖性;尼美舒利可诱导食管鳞癌细胞凋亡,可抑制前列腺素E2的合成。结论尼美舒利抑制食管鳞癌细胞增生诱导凋亡作用,可能是通过抑制前列腺素E2的合成而实现的。环氧化酶-2抑制剂有可能在食管鳞癌的预防及治疗上发挥重要作用。

Effects of Proliferation Inhibition and Apoptosis Induced by COX-2 Selective Inhibitor on Esophageal Squamous Carcinoma Cells

Objective To investigate the effects of nimesulide(a non-selective cyclooxygenase-2 inhibitor) on cell proliferation inhibition and apoptosis in esophageal squamous cell carcinoma(ESCC) cell line.Methods EC109 cells were incubated with nimesulide.Cell proliferation was assessed by MTT assay.Cell apoptosis induction were determined by fluorescence staining flow cytometry.PGE_(2) production was measured by ELISA assay to determine the effect of nimesulide on cyclooxygenase activity.Results Nimesulide inhibited cell growth in a concentration and time dependent manner.Apoptosis was induced by Nimesulide dose dependently.This was accompanied with a decrease of PGE_(2) production.Conclusion Nimesulide could inhibit ESCC cell proliferation and induce apoptosis,which is probably through cyclooxygenase-2 inhibition.Cyclooxygenase-2 inhibitor may be a promising therapeutic agent for human ESCC.