甲状腺激素对大鼠急性肝衰竭肠粘膜屏障功能的保护作用

目的探讨补充外源性甲状腺激素(thyroid hormone,TH)对急性肝衰竭(acute hepatic failure,AHF)肠粘膜屏障的保护作用.方法 SD大鼠36只,随机分为3组:对照组(SO组)、急性肝衰竭组(AHF组)和治疗组(AHF+TH组).腹腔注射硫代乙酰胺(thioacetamide,TAA)制作AHF模型.15μg/kg的标准腹腔注射三碘甲状腺原氨酸(T3),取下腔静脉血检测TH水平,取小肠组织测二胺氧化酶(DAO)活性,取末段回肠行普通HE染色观察,并使用图像分析系统测量小肠绒毛长度.结果 AHF+TH组较AHF组小肠组织DAO活性升高(P<0.05),小肠绒毛长度升高(P<0.05),肠粘膜损伤程度降低(P<0.05).结论补充外源性TH对AHF时肠粘膜屏障有明显的保护作用.

The Protective Effct of Thyroid Hormone on Function of Intestinal Mucosa Barrier in Rats with Acute Hepatic Failure

Objective To investigate the injury of intestinal mucosa barrier,the metabolic abnormalities of thyroid hormone,and the protective effect of exogenous thyroid hormone on intestinal mucosa barrier in rats with acute hepatic failure.Methods 36 SD rats were randomly divided into 3 groups: control group(SO group),acute live failure group(AHF group)and treatment group(AHF+TH group).The AHF Rat model was established by repeated injection of thioacetamide(TAA).TH was administered intraperitoneally to correct the low TH syndrome of AHF rats.The blood in inferior vena cava was collected to detect the level of thyroid hormone and liver function,the blood in portal vein was collected to detect the level of endotoxin,and the liver was sampled to observe the pathological structure by HE staining,the length of intestinal villi was determined by image analysis system.Results Compared with AHF group,the liver function of rats in AHF+ TH group was significantly improved(P<0.05),the blood levels of endotoxin were decreased and the length of intestinal villi was extended(P<0.05).Conclusion The supplement of exogenous thyroid hormone can protect intestinal mucosa barrier and the function of liver,and alleviate the injure of intestinal mucosa barrier in rats with acute hepatic failure.