附子HPLC数字化指纹图谱研究

目的建立附子HPLC数字化指纹图谱。方法采用反相高效液相色谱法,以Century SILC18 AQ色谱柱,0.1mol.L-1NaH2PO4水溶液-甲醇为流动相,低压线性梯度洗脱,流速：1.0mL·min^-1,柱温：（30±0.15）℃,检测波长：280nm。用＂中药色谱指纹图谱超信息特征数字化评价系统3.0＂软件从分离度、信息量、定性定量相似度等6个方面对不同批次的附子HPLC指纹图谱进行潜信息特征数字化评价。结果以鸟苷峰为参照物峰,确定了41个共有峰,建立了四川江油地区附子的HPLC数字化指纹图谱。应用色谱指纹图谱指数F等参数对不同产地附子HPLC指纹图谱的超信息特征进行了数字化评价,获得了判别附子质量的重要数字信息,以双定性双定量相似度法评价附子批间质量稳定性。结论所建立的HPLC指纹图谱具有较好的精密度和重现性,适用于附子药材的信息质量控制。

Digitized fingerprints of Radix Aconiti Lateralis Preparata by HPLC

Objective To establish the HPLC digitized fingerprints of Radix Aconiti Lateralis Preparata. （RALP）. Methods The chromatographic fingerprints were developed using CenturySIL C18 AQ as the separation column and 0. 1 mol · L^-1NaH2PO4-methanol as the mobile phase. The flow rate was 1.0 mL · min^-1 and the detection wavelength was 280 nm. Results Forty-one co possessing fingerprint peaks were selected as the fingerprints of RALP by taking guanosine peak as the referential peak to establish the digitized HPLC fingerprint, and the important digitized information was obtained for its quality control. Indexes such as chromatographic fingerprint index F were used to evaluate the HPLC fingerprints. The stabilities among different batches of samples were evaluated by the dual qualitative similarities and dual quantitative similarities. Conclusion This method is precise and reproducible, and can be useful in the information quality control of RALP.