| 半夏丛生芽诱导及快速繁殖 |
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| 引用本文： | 徐秀梅,吕道锡,孙敏,张明,陈敏.半夏丛生芽诱导及快速繁殖[J].中药材,2005,28(12):1052-1053. |
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| 作者姓名： | 徐秀梅 吕道锡 孙敏 张明 陈敏 |
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| 作者单位： | 1. 西南大学生命科学学院,重庆,400715
2. 重庆市中药研究院,重庆,400065 |
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| 基金项目： | 国家科技基础条件平台工作项目，重庆市科委科研项目 |
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| 摘 要： | 目的:以叶片、叶柄为外植体,建立半夏的快速繁殖体系.方法:用半夏块茎诱导的丛生芽叶片、叶柄作外植体.结果:叶片、叶柄可以不经过愈伤组织而直接再生成植株,培养基MS 6-BA 2.0 mg/L NAA 0.1 mg/L对外植体叶的分化诱导效果最好,MS 6-BA 2.0 mg/L NAA 0.25 mg/L则适于叶柄,生根培养基以1/2MS KT 1.0 mg/L NAA 2.0 mg/L效果较好.结论:通过对半夏块茎诱导的叶片和叶柄形成的丛生芽可以提高半夏繁殖系数.
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| 关 键 词： | 半夏 叶片 叶柄 丛生芽 快速繁殖 |
| 收稿时间： | 2005-05-24 |
| 修稿时间： | 2005-09-20 |
Clustered Shoot Induction and Rapid Propagation on Pinellia ternate |
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| Xu Xiumei,Lu Daoxi,Sun Min,Zhang Ming,Chen Min.Clustered Shoot Induction and Rapid Propagation on Pinellia ternate[J].Jorunal of Chinese Medicinal Materials,2005,28(12):1052-1053. |
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| Authors: | Xu Xiumei Lu Daoxi Sun Min Zhang Ming Chen Min |
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| Institution: | 1. School of Life Sciences, Southwest University, Chongqing 400715 ; 2. Institute of Chinese Medicine, Chongqing 400065 |
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| Abstract: | Object: To establish the rapid propagation system of Pinellia ternate. Method: The Clustered shoots were induced by the explant lamina and petiole from the terminal bud of Pinellia ternate tuber in test tube. Result: The plantlets could regenerate from the lamina and petiole directly without via the callus. The MS Medium with 2.0 mg/L 6-BA and 0.1 mg/L NAA was optimal for the induction of culstered shoots from explants of lamina; the MS with 2.0 mg/L 6-BA and 0.25 mg/L NAA was best for the explants of petiole; and the 1/2 MS with 1.0 mg/L KT and 2.0 mg/L NAA was the better medium for induction of roots. Conclusion:The regenerated rate of Pinellia ternata can improve by inducing the clustered shoots from the explants of lamina and petiole directly. |
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| Keywords: | Pinellia ternate Lamina Petiole Clustered shoots Rapid propagation |
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