老年大鼠空间记忆与海马N-甲基-D天冬氨酸受体2B亚基mRNA的表达

目的 观察老年大鼠在Y迷宫训练建立空间记忆后N-甲基-D-天冬氨酸受体(NMDA)2B亚基mRNA在海马区的表达变化.方法 用Y迷宫对老年(12月龄)大鼠进行短期强化训练,建立空间记忆后,于即刻,3h,6h,12h,24h,3d每组处死8只老年大鼠,取双侧海马,应用RT-PCR方法 检测大鼠海马NM-DA受体2B亚基(NR2B)mRNA的表达变化.结果 训练组动物(包括即刻,3h,6h,12h,24h组)海马NR2B mRNA的表达量分别为(683.83±78.13),(806.00±81.43),(848.83±80.84),(915.17±84.25),(568.53±77.14)]明显高于对照组和假训练组(398.17±74.76),(418.33±70.17),P<0.05],且12h时表达最多.3d组的表达量(430.33±74.41)与对照组和假训练组相比差异无统计学意义.结论 NR2B可能参与了学习记忆信号的转导过程,且在记忆训练后12h时表达最多,3d时恢复到基础水平.

The changes of NMDA receptor 2B subunit expression in hippocampus of the old rats after establishment of spatial memory

Objective To observe the changes of NMDA receptor 2B mRNA expression in hippocampus of the old rats after establishment of spatial memory.Methods The spatial memory was establishment by reinforcing training in the old rats(12 months).Then the animals were killed eight rats every group at the immediate ,3,6,12,24h and 3d after establishment of spatial memory and the two hippocampus were isolated for determination of mRNA of NMDA receptor 2B subunit(NR2B) expression using RT-PCR technique.Results The training groups (including instant ,3h,6h,12h,24h groups) showed significantly higher expressions of NR2B (683.83±78.13,806.00±81.43,848.83±80.84,915.17±84.25,568.53±77.14) than control group and pseudotraining group (398.17±74.76,418.33±70.17)(P<0.05).AT the moment of 12h got the maximum expression.There were not significantly different between 3d group and control and pseudotraining group.Conclusion NR2B maybe participate in the signal transduction during learning and memory processes ,and got the most at 12h then recovered after 3d.