A simple immunofluorescence assay for C1q binding |
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| Authors: | Ewert Linder |
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| Affiliation: | Clinical Immunology Section, Department of Bacteriology and Immunology, University of Helsinki, SF-00290 Helsinki 29, Finland |
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| Abstract: | ![]()
Antibody-independent binding of C1q to Crithidia luciliae kinetoplast DNA was demonstrated by immunofluorescence microscopy. The method allows detection of C1q in human sera diluted 1/20–1/40 and in 5–10 μg/ml concentrations of isolated C1q. Various substances known to interfere with the early events of complement activation inhibited binding of C1q to kinetoplasts. The method may be used as a functional assay for serum C1q. |
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