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Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein |
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| Authors: | Yean-Kai Tsung Aubrey Milunsky Elliot Alpert |
| Affiliation: | 1. Genetics Division, Eunice Kennedy Shriver Center, Waltham, MA 02154, USA;2. Gastrointestinal Unit, Medical Service, Massachusetts General Hospital, Boston, MA, USA;3. Department of Pediatrics and Medicine, Harvard Medical School, Boston, MA, U.S.A. |
| Abstract: | A monoclonal antibody specific for human alpha-fetoprotein (HAFP) was derived by the hybridoma technique. Spleen cells from mice immunized with pure HAFP were fused with a mouse myeloma cell line (P3×63-Ag-8) and an anti-HAFP secreting hybridoma cell line was cloned in soft agarose. The HAFP specific antibody was shown to be a monoclonal IgG1 subclass with extremely high avidity for HAFP. |
| Keywords: | Address for reprints: Dr. Aubrey Milunsky Genetics Division Eunice Kennedy Shriver Center 200 Trapelo Road Waltham MA 02154 U.S.A. |
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