HPLC同时测定木香分气丸中柚皮苷、橙皮苷和新橙皮苷的含量

目的:建立木香分气丸中柚皮苷、橙皮苷和新橙皮苷的含量测定方法.方法:采用反相HPLC,色谱柱为Agilent C18柱(4.6 mm×250 mm,5μm),流动相乙腈-1％磷酸水(21∶79),流速1.0 mL·min-1,检测波长283 nm,柱温30℃.结果:柚皮苷进样量在0.1108～1.2188 μg线性关系良好(r=0.999 4,n=6),平均回收率为97.5％(RSD 0.80％,n=6);橙皮苷进样量在0.226 8 ～2.494 8μg线性关系良好(r =0.999 7,n=6),平均回收率99.0％ (RSD 1.7％,n=6);新橙皮苷进样量在0.084 8～0.932 8μg线性关系良好(r=0.999 4,n=6),平均回收率为98.2％ (RSD1.6％,n=6).结论:该方法重复性好、灵敏度高、定量准确,可作为木香分气丸质量控制方法之一.

Determination of Narigin, Hesperidin and Neohesperidin in Muxiang Fenqi Pill by HPLC

Objective:To develop an HPLC method for determination of narigin,hesperidin and neohesperidin in Muxiang Fenqi Pill.Method:The analysis was carried out with an Agilent C18(4.6 mm×250 mm,5μm)column and the mobile phase of acetonitrile-1% phosphoric acid(21∶79).The flow rate was 1.0 mL · min-1 and column temperature was 30 ℃.The detection wavelength was set at 283 nm.Result: The calibration curve was linear within the range of 0.110 8-1.218 8 μg(r=0.999 4,n=6),0.226 8-2.494 8 μg(r=0.999 7,n=6)and 0.084 8-0.932 8 μg(r=0.999 4,n=6) for narigin,hesperidin and neohesperidin.The average recoveries were 97.5%(RSD 0.80%,n=6),99.0%(RSD 1.7%,n=6) and 98.2%(RSD 1.6%,n=6) respectively.Conclusion:The results showed this method is reproducible,sensitive and accurate for determination of narigin,hesperidin and neohesperidin in Muxiang Fenqi Pill.