慢性髓系白血病急变期与急性白血病免疫表型的比较研究

目的比较慢性髓系白血病急变期（CML-BC）与急性白血病（AL）的免疫表型特征。方法采用一组单克隆抗体和四色流式细胞术对58例成人CML-BC及同期420例AL患者骨髓标本进行免疫表型分析。结果58例CML-BC中。CML-AML占77．6％，CML-ALL占13．8％，CML-MAL（混合性白血病）占5．2％；AL中，AML占66．2％。ALL占32．9％，MAL占1．0％。CML-AML中，CD7及CD34表达高于AML；CML-BC和CML-AML中CD7与CD34共阳性表达分别高于AL和AML（P〈0．05）。276例AML中，纯型占62．3％，变异型占37．7％；130例ALL中，纯型占44．6％，变异型占55．4％；42例CML-AML中。纯型占59．5％，变异型占40．5％；8例CML-ALL中，纯型与变异型各占50．0％。结论CML-BC中干祖细胞阶段的抗原较AL高表达，变异型CML-BC与AL比例相当。

Comparative Study of the Immunophenotype of Chronic Myeloid Leukemia in Blast Crisis and Acute Leukemia

Objective To compare the immunophenotypic features of chronic myeloid leukemia(CML) in blast crisis and acute leukemia(AL).Methods Immunophenotypic analysis was performed using a panel of monoclonal antibodies and four-color immunofluorescence staining methods of flow cytometry in 58 patients with CML-BCLL and 420 patients with AL during the same period.Results 77.6% of the CML-BC patients were AML transformation,13.8% was ALL transformation and(5.2%) was MAL(mixed lineage acute leukemia) transformation.In AL,66.2% of the patients were AML,32.9% was ALL and 1.0% was MAL.In CML-AML,CD34 and CD7 positivity was higher than that in AL.Coexpression of CD7 and CD34 was more frequent in CML-BC and CML-AML than that in AL and AML(P<0.05) respectively.In 276 AMLcases,the pure phenotype accounted for 62.3% and variant type 37.7%.In 130 ALLcases,the pure phenotype accounted for 44.6% and variant type 55.4%.The pure and variant type accounted for 59.5% and 40.5% in 42 cases of CML-AML,50.0% and 50.0% in 8 cases of CML-ALL.Conclusions The present study suggests that the hematopoietic precursor antigen is more frequent in CML-BC than that in AL.The variant type is proper in CML-BC and AL.