三七总皂苷对异丙肾上腺素致大鼠心肌肥厚的保护作用

目的:研究三七总皂苷(PNS)对异丙肾上腺素(isoproterenol,ISO)所致大鼠心肌肥厚的保护作用.方法:5mg/kg·d连续7d建立大鼠心肌肥厚模型.造模第二天开始ip PNS 25和50 mg/kg·d连续14d,测定全心重量指数(HW/BW)、左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量,及Na ,K -ATP酶和Ca2 -ATP酶活性;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量.结果:ISO模型组大鼠的HW/BW、LVI明显升高;左心室Hyp、AngⅡ含量增高,NO水平下降,Na ,K -ATP酶和Ca2 -ATP酶活性降低.PNS能明显提高心肌组织NO水平及Na ,K -ATP酶和Ca2 -ATP酶活性,降低胶原的含量,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚.结论:PNS对ISO所致大鼠心肌肥厚具有保护作用.

Protective effects of Total Saponins of Panax notoginseng on myocardial hypertrophy induced by isoproterenol in rats

Objective: To investigate the protective effects of total saponins of panax notoginseng (PNS) on myocardial hypertrophy induced by isoproterenol (ISO) in rats. Methods: Myocardial hypertrophy model of rats were induced by injection of ISO 5mg/kg.d sc for 7d. From day 2, the rat were administered with PNS 25 and 50mg/kg.d ip for 14d,the control and ISO model group were received saline injection.Then, the heart-weight (HW),left ventricular weight (LVW), the ratio of HW/BW and LVW/BW (LVI) were measured; the content of hydroxyproline and angiotensin (AngII) , the level of nitric oxide(NO) and the activity of Na+,K+-ATPase and Ca 2+ -ATPase in left ventricle were determined by spectrophotometry and radioimmunoassay respectively. Results: In ISO model group, HW/BW, LVI and the content of hydroxyproline and AngII in left ventricle markedly increased; the level of NO and the activity of Na+,K+-ATPase, Ca 2+ -ATPase in the left ventricle were decreased. Treatment with PNS significantly reduced the HW/BW,LVI, decreased the AngII and the hydroxyproline content, increased the level of NO and the activity of Na+,K+-ATPase and Ca 2+ -ATPase. Conclusion: PNS was shown to prevent remodeling of myocardial hypertrophy induced by ISO in rats.