藤黄酸诱导胃癌细胞凋亡及抗肿瘤转移的实验研究

目的研究藤黄酸对人胃癌BGC-803细胞增殖和转移相关能力的影响及其机制。方法 MTT、Hoechst染色法检测藤黄酸对BGC-803细胞的增殖和凋亡的影响;侵袭小室模型等方法检测藤黄酸对BGC-803细胞侵袭、黏附、迁移的影响;运用RT-PCR、Western-blotting法分析藤黄酸对胃癌细胞凋亡和转移相关基因bcl-2、bax、细胞黏附分子-1(ICAM-1)mRNA和蛋白表达的影响。结果 藤黄酸抑制BGC-803细胞的生长,其抑制率与药物浓度及作用时间呈依赖关系。低浓度的藤黄酸处理BGC-803细胞后,可明显降低胃癌细胞的黏附、迁移和侵袭能力。随着藤黄酸浓度的增大,bcl-2I、CAM-1 mRNA和蛋白表达均下调,bax mRNA和蛋白表达上调。结论藤黄酸对胃癌细胞BGC-803有明显的生长抑制作用,具有显著的促肿瘤细胞凋亡作用和抑制肿瘤细胞转移相关性质,其机制可能与下调bcl-2I、CAM-1及上调bax的表达有关。

Experimental study on gambogic acid-induced gastric cancer cell apoptosis and anti-tumor metastasis

Objective To investigate the inhibition of gambogic acid on proliferation and metastasis ability of BGC-803 cells and to study their preliminary mechanism further.Methods Methyl thiazolyl tetrazolium(MTT) and Hoechst staining were used to study the effects of gambogic acid-induced apoptosis in gastric cancer cell line BGC-803;Invasive methods were used to study the effects of invasion,adhesion,migration by gambogic acid;The influence of gambogic acid on the expression of bcl-2,bax,and ICAM-1 gene in gastric cancer cells was evaluated by RT-PCR and Western-blotting.Results Gambogic acid was found not only to inhibit the growth of BGC-803 cells but also to induce the cell apoptosis in a dose-and time-dependent manner.The ability of adhesion,migration,and invasiveness was clearly inhibited by gambogic acid.With the increase of gambogic acid concentration,the expression of bcl-2,ICAM-1 gene,and protein were down-regulated and the expression of bax gene and protein were up-regulated.Conclusion Gambogic acid could inhibit the growth of gastric cancer cells BGC-803 significantly,have a significant role in promoting tumor cell apoptosis,and inhibit tumor cell metastasis-associated properties.The mechanism may be related to decrease of bcl-2,ICAM-1 expression and increase bax expression.