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| 低氧条件刺激胃上皮细胞株GES-1后MICA表达研究 | |
| 引用本文： | 蒲小燕,张晓岩,杨生玺. 低氧条件刺激胃上皮细胞株GES-1后MICA表达研究[J]. 山东医药, 2010, 50(32): 10-11 |
| 作者姓名： | 蒲小燕 张晓岩 杨生玺 |
| 作者单位： | 青海大学医学院,西宁,810001 |
| 基金项目： | 青海省科技厅重点科技攻关项目 |
| 摘 要： | 目的研究低氧对体外培养胃上皮细胞株GES-1中MHC-Ⅰ类链相关蛋白A（MICA）基因表达的影响。方法建立GES-1细胞体外低氧培养模型,免疫细胞化学技术分别检测MICA在缺氧处理0、24、72 h后的蛋白表达。结果免疫细胞化学证实MICA蛋白的表达随着缺氧时间的增加呈时间效应关系,且表达量随缺氧时间而升高（P〈0.05）。结论低氧可以显著增加胃上皮细胞株GES-1中MICA的表达。 |
| 关 键 词： | 低氧 MHC-Ⅰ类链相关蛋白A 胃上皮细胞株GES-1 |
Effects of hypoxia on expression of MICA in human gastric epithelial cell strain-1 |
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| PU Xiao-yan,ZHANG Xiao-yan,YANG Sheng-xi. Effects of hypoxia on expression of MICA in human gastric epithelial cell strain-1[J]. Shandong Medical Journal, 2010, 50(32): 10-11 | |
| Authors: | PU Xiao-yan ZHANG Xiao-yan YANG Sheng-xi |
| Affiliation: | (Medical College of Qinghai University,Xining 810001,P.R.China) |
| Abstract: | Objective To investigate the effect of hypoxia on major histocompatibility complex class Ⅰ chainrelated protein A（MICA） expression in gastric epithelial cell strain-1（GES-1） and its mechanism.Methods Hypoxia culture model in vitro was established to test the expression of MICA in GES-1 cell line.MICA expression was tested in protein level by using immunocytochemistry analysis after cultured in hypoxia circumstance for 0,24,and 72 hours,respectively.Results MICA expressions increased after treatment of hypoxia with time-effect relationship（P〈0.05）.Conclusion MICA expression in GES-1 can be induced remarkably by hypoxia. |
| Keywords: | hypoxia MICA gastric epithelial cell strain-1 |
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