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| 甲状旁腺激素对人牙乳头细胞牙本质基质蛋白1表达的影响 | |
| 引用本文： | 逄键梁,吴补领,张亚庆,何文喜,郭鹏,高杰. 甲状旁腺激素对人牙乳头细胞牙本质基质蛋白1表达的影响[J]. 牙体牙髓牙周病学杂志, 2003, 13(7): 372-375 |
| 作者姓名： | 逄键梁 吴补领 张亚庆 何文喜 郭鹏 高杰 |
| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
| 基金项目： | 由全军“十五”重点课题资助 (编号 0 1Z0 89) |
| 摘 要： | 目的：观察甲状旁腺激素(parathyroid hormone，PTH)对体外培养的人牙乳头细胞(human dental papilla cells，HDPC)牙本质基质蛋白1(dental matrix protein，DMP1)合成分泌的影响。方法：原代方法培养出人牙乳头细胞，用不同浓度的甲状旁腺激素刺激培养的第5代人牙乳头细胞，免疫组化观察结果，并采用图像分析的方法，进行半定量分析。结果：PTH可刺激人牙乳头细胞DMP1的表达，诱导的DMP1主要表达于细胞胞浆内，呈一定的浓度依赖性，0．3μg／mL为刺激最佳浓度。结论：PTH能够刺激人牙乳头细胞产生DMP1，对牙乳头细胞向成牙本质细胞分化有一定促进作用，可能是成牙本质细胞分化的重要介质之一。 |
| 关 键 词： | 人牙乳头细胞 甲状旁腺激素 牙本质基质蛋白1 |
| 文章编号： | 1005-2593(2003)07-0372-04 |
| 修稿时间： | 2002-10-21 |
Effect of PTH on the expression of DMP1 in human dental papilla cells in vitro |
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| PANG Jian-liang,WU Bu-ling,ZHANG Ya-qing,et al. Effect of PTH on the expression of DMP1 in human dental papilla cells in vitro[J]. Chinese Journal of Conservative Dentistry, 2003, 13(7): 372-375 | |
| Authors: | PANG Jian-liang WU Bu-ling ZHANG Ya-qing et al |
| Abstract: | AIM:To study the effect of parathyroid hormone (PTH) on the expression of dental matrix protein 1(DMP1)in cultured human dental papilla cells(HDPC).METHODS:Immunohistochemical staining was done on HDPC derived of primary culture on stimulation from different doses of PTH,and difference of the amount of DMP1 expression was determined by Medical Image Analysis System.RESULTS:The expression of DMP1 was majorly found in HDPC plasms on stimulation from PTH,and the effect of PTH on the expression of DMP1 was related to the concentration of PTH.CONCLUSION:These results suggested that PTH may be involved in the expression of DMP1 in HDPC in vitro and it may act as one of mediators in the differentiation of odontoblasts. [ |
| Keywords: | human dental papilla cells parathyroid hormone dental matrix protein 1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |