行辅助生殖技术患者流产胎儿人白细胞抗原-G基因14bp插入/缺失多态性分析

目的探讨行辅助生殖技术后流产但染色体数目正常的胎儿人白细胞抗原-G(HLA-G)基因第8外显子14bp插入/缺失多态性情况,以期揭示其与流产发生的相关性。方法应用聚合酶链反应(PCR)及片段分析方法对符合入选条件的47例体外受精(IVF)流产胎儿(IVF流产组)、35例卵胞浆内单精子注射(ICSI)流产胎儿(ICSI流产组)及49例自然妊娠但选择行人工流产的胎儿(染色体数目正常;对照组)的HLA-G基因14bp插入/缺失多态性进行分析。结果 14bp插入/缺失等位基因频率在各组间相近,无统计学差异(P0.05);各组的HLA-G基因+14/+14、+14/-14和-14/-14基因型频率相似,无统计学差异(P0.05)。结论结果提示HLA-G基因14bp插入/缺失多态性不是引起通过辅助生殖技术方式妊娠的染色体数目正常胎儿流产发生的原因。

Analysis of the 14 bp insertion/deletion polymorphisms of human leukocyte antigen-G gene in abortuses conceived through assisted reproductive technology

Objeetive： To explore the correlation between miscarriage and the polymorphism of human leukocyte antigen-G （ H LA-G） gene. Methods： The 14 bp insertion/deletion polymorphisms in the eighth exon of HLA-G gene in the abortuses with normal chromosome number, which conceived through assisted reproductive technology, were investigated. Forty-seven abortuses from IVF,thirty-five abortuses from ICSI and forty-nine elective termination fetuses were collected. The chromosome number of all the abortuses or fetuses was normal and met our inclusion criteria. The tissues of abortuses and fetuses were used for analyzing HLA-G gene 14 bp insertion/deletion polymorphisms with PCR and fragment analysis methods. Results： The allele frequencies of HLA-G＋14 bp and HLA-G -14 bp were not significant different in all study group（P〈0.05）. The genotypic frequencies of＋ 14/＋ 14, ＋ 14/-14 and -14/-14 were also not significant different in all study group（P〈0.05）. Conclusions. Our results suggest that the 14 bp insertion/deletion polymorphisms of HLA-G gene should not responsible for the origin of miscarriage in abortuses conceived through assisted reproductive technology with normal chromosome number.