| RP-HPLC测定不同产地白花丹中白花丹醌的含量 |
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| 引用本文： | 刘圆,刘超. RP-HPLC测定不同产地白花丹中白花丹醌的含量[J]. 中国药学杂志, 2007, 42(18): 1429-1430 |
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| 作者姓名： | 刘圆 刘超 |
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| 作者单位： | 西南民族大学少数民族药物研究所,成都,610041 |
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| 基金项目： | 四川省青年基金;四川省科技攻关项目;四川省自然科学基金;四川省教育厅教育教学改革项目 |
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| 摘 要： | 目的分析不同产地白花丹药材中白花丹醌含量的差异。方法采用Kromasil C18柱(4.6mm×200mm,5μm),柱温:30℃,流动相:甲醇-水(65∶35),检测波长:213nm,流速:1mL·min-1。结果白花丹醌在0.0104～0.3328μg,峰面积与进样量具有良好的线性关系,回归方程为Y=-22.32+9527.9X,r=0.9999,平均回收率为98.7%。结论本法简便、准确、重复性好,可用于控制白花丹药材质量。
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| 关 键 词： | 白花丹 白花丹醌 含量 RP-HPLC |
| 文章编号： | 1001-2494（2007）18-1429-02 |
| 收稿时间： | 2006-08-22; |
| 修稿时间： | 2006-08-22 |
Determination the Content of Plumbagin in Plumbago zeylanica L.from Different Areas by RP-HPLC |
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| LIU Yuan,LIU Chao. Determination the Content of Plumbagin in Plumbago zeylanica L.from Different Areas by RP-HPLC[J]. Chinese Pharmaceutical Journal, 2007, 42(18): 1429-1430 |
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| Authors: | LIU Yuan LIU Chao |
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| Affiliation: | Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chendu 610041, China |
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| Abstract: | OBJECTIVE To determine the Plumbagin in Plumbago zeylanica from different areas.METHODS A RP-HPLC method was established using a kromasil C18 column (4.6 mm×200 mm,5 μm).The mobile phase was methanol-water(65∶35). The flow rate was 1 mL·min-1 with UV detection at 213 nm. The column temperature was 30 ℃.RESULTS The method displayed good linearity within the concentration ranges of 0.010 4～0.332 8 μg (r=0.999 9).The average recovery was 98.7%.CONCLUSION This method is simple, accurate, reproducible and suitable for the determination of Plumbagin in P. zeylanica L. |
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| Keywords: | RP-HPLC |
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