利用雌激素构建小鼠淋病奈瑟菌感染模型

目的探讨用雌激素构建淋病奈瑟菌感染小鼠模型的方法。方法淋病奈瑟菌WHO-A接种经皮下注射苯甲酸雌二醇和真皮下埋植尼尔雌醇片（雌三醇）预处理的雌性ICR小鼠，小鼠阴道拭子接种T-M选择培养基培养分离淋病奈瑟菌，取小鼠阴道分泌物涂片染色观察淋病奈瑟菌感染情况，比较两种雌激素对小鼠淋病奈瑟菌感染的差异。结果与对照组相比，苯甲酸雌二醇小鼠体内淋病奈瑟菌有短时间的定植，而尼尔雌醇组与对照组无差异。结论雌激素（尤其是雌二醇）有利于淋病奈瑟菌在小鼠阴道内的定植。

Model Establishment of Neisseria gonorrhoeae Infection in Mouse by Using Estrogen

Objective To find out a method of constructing a Neisseria gonorrhoeae genital infection mouse model.Method In our experiments,ICR mice were injected Estradiol(E2) and inplanted Estriol(E3) pellet respectively,then challenged with Neisseria gonorrhoeae WHO-A in the female mice vagina.The genital swab were verified by T-M culture media,the vaginal excretes smears were stained to determine different effect of the hormone on mouse Neisseria gonorrhoeae infection.Result Compared with non-estrogen-treated mice,estradiol(E2) treated mice have Neisseria gonorrhoeae short-lived in the vagina.And there was no gonococci in estriol(E3) treated mice like control group.Conclusion Estrogen,especially estradiol(E2) has the effect on promoting Neisseria gonorrhoeae vaginal colonization in mice.