MMP-9、TIMP-1在大鼠视网膜缺血-再灌注损伤中的表达

目的 探讨高眼压视网膜缺血-再灌注(retina ischemia-reperfusion，RIR)损伤时大鼠视网膜基质金属蛋白酶9(matrix metalloproteinase 9，MMP-9)和组织金属蛋白酶抑制剂1(tissue inhibitor of metalloproteinase 1，TIMP-1)的动态变化及意义。方法 利用前房高压灌注法造成大鼠视网膜缺血1h，再恢复其供血，并于再灌注0h、3h、12h、24h、48h和72h后处死，采用免疫组化SP法和计算机图像分析系统检测视网膜MMP-9和TIMP-1的表达和分布。结果 RIR 0h视网膜MMP-9表达极弱，MMP-9阳性染色于RIR3h出现，12h迅速增加，24h达高峰，以后逐渐下降，72h接近正常。RIR各时段TIMP-1水平无明显改变。结论 MMP-9是参与RIR损伤的重要因素．

Expression of MMP-9 and TIMP-1 in experimental retinal ischemia-reperfusion injury in rats

Objective To investigate the dynamic changes and significance of matrix metalloproteinase 9(MMP-9) and tissue inhibitor of metalloproteinase 1(TIMP-1) in an experimental retinal ischemia-rperfusion(RIR) injury.Methods Ischemia injury was induced in adult rats by increasing the prssure of the anterior chamber with perfusion of normal saline into anterior chamber.After 1 hour of ischemia and a varied reperfusion time (0 hour,3 hours,12 hours,24 hours,48 hours and 72 hours),the rat eyes were enucleated.The levers of MMP-9 and TIMP-1 and their distribution in retina were confirmed by immunohistochemistry and analyzed by computer-picture analytic system.Results MMP-9 positive expression was rarely detected in the control and RIR 0 hour specimens,while it appeared at RIR 3 hours,increased markedly at 12 hours,peaked at 24 hours,then dropped gradually afterwards and decreased to the baseline level at 72 hours.TIMP-1 remained relatively unaltered in various groups.Conclusion MMP-9 may play an important role in the pathomechamism of RIR injury.