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| 鹿属动物线粒体DNA序列测定的研究 | |
| 引用本文： | 傅文,唐双焱,陈永久. 鹿属动物线粒体DNA序列测定的研究[J]. 中国药学杂志, 2000, 35(12): 803-804 |
| 作者姓名： | 傅文 唐双焱 陈永久 |
| 作者单位： | [1]云南省药品检验所,云南昆明 [2]中国科学院昆明动物研究所,云南昆明 |
| 基金项目： | 国家自然科学基金!资助项目 (3976 0 0 81) |
| 摘 要： | 目的 通过DNA序列测定，寻找鹿属动物线粒体DNA的特异性片段，用于鹿类药材的鉴定。方法 对9种鹿属动物的线粒体DNA进行引物扩增和序列测定，并与牛科、马科的DNA序列进行比较。结果 找到了鹿属动物特异的DNA序列。结论 利用该序列可对鹿类药材进行真伪区分。 |
| 关 键 词： | 鹿类动物线粒体DNA 序列测定 |
| 收稿时间： | 2000-05-31; |
| 修稿时间： | 2000-05-31 |
Research on the sequencing of mitochondrial DNA of deer genus |
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| FU Wen,TANG Shuang yan,CHEN Yong jiu,WANG Jian yun,JIANG Xu,ZHANG Ya ping. Research on the sequencing of mitochondrial DNA of deer genus[J]. Chinese Pharmaceutical Journal, 2000, 35(12): 803-804 | |
| Authors: | FU Wen TANG Shuang yan CHEN Yong jiu WANG Jian yun JIANG Xu ZHANG Ya ping |
| Abstract: | OBJECTIVE To search the segments of mtDNA of deer genus by sequencing and to identify the traditional Chinese medicine of deer.METHOD The segments of mtDNA of 9 samples of deer were amplified by PCR technique with common primes and sequenced by the DNA auto sequence machine.RESULTS Compared with the sequences of mtDNA of bovine and horse,the distinctive characteristics of sequences of deer genus were found.CONCLUSION This method appeared to be valuable for identifing the traditional Chinese medicine of deer. |
| Keywords: | mitochondrial DAN of deer genus sequence identificationd |
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