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| 大鼠脑微血管内皮细胞的培养与鉴定 | |
| 引用本文： | 孙文萍,汤雪晴. 大鼠脑微血管内皮细胞的培养与鉴定[J]. 医学综述, 2008, 14(23): 3660-3662 |
| 作者姓名： | 孙文萍 汤雪晴 |
| 作者单位： | 1. 北华大学附属医院特需科,吉林,吉林,132011 2. 成都军区总医院儿科,成都,610081 |
| 摘 要： | 目的探讨大鼠脑微血管内皮细胞体外培养方法,为血管内皮细胞的研究打下基础。方法用胶原酶消化大鼠脑皮质制成细胞悬液再进行培养,用光镜及免疫组织化学检测进行鉴定。结果分离的大鼠脑微血管内皮细胞体外培养5～7d左右可长成单层,光镜下为多角形或扁平梭形,呈"铺路石样"排列,第Ⅷ因子相关抗原免疫组织化学测定阳性。结论该分离细胞培养法可培养出大鼠的脑微血管内皮细胞。 |
| 关 键 词： | 脑微血管 内皮细胞 体外培养 |
The Methods of Culturing and Identifying Cerebra Lmicrovascular Endothel Cell in Rats |
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| SUN Wen-ping,TANG Xue-qing. The Methods of Culturing and Identifying Cerebra Lmicrovascular Endothel Cell in Rats[J]. Medical Recapitulate, 2008, 14(23): 3660-3662 | |
| Authors: | SUN Wen-ping TANG Xue-qing |
| Affiliation: | 1.Department of Special Procurement Ward;the Affiliated Hospital of Beihua University;Jilin 132011;China;2.Department of Pediatrics;General Hcspital of Chengdu Military Region;Chengdu 610081;China |
| Abstract: | Objective To explore the methods of culturing rat cerebral microvascular endothelial cells (RCMECs),so as to build up the basis for the studies of vascular endothelial cells.Methods After the RCMEC were digested with collagenase:to make suspension which are cultivated,the RCMEC were identified by immunohistochemical methods and observed with light microscopeandelectron microscope.Results Isolated RCMECs grow tomonolayer after 5~7 days of external cultivation.They present polygonal and arrange like pavement ... |
| Keywords: | Cerebral microvascular Endothelialcell Cultivation inexternal |
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