用免疫印迹法测定肺表面活性蛋白A及其临床应用

肺表面活性蛋白Ａ含量测定可作为胎儿肺发育成熟的指标和肺部疾病的辅助诊断，以及用于疗效观察和预后估计。本文建立了免疫印迹分析ＳＰＡ法，测定了５５例３６￣４２孕周健康产妇羊水中ＳＰＡ含量，发现随着孕周增加，ＳＰＡ含量呈上升趋势。本文还测定了２６例儿胸外科手术病人术前和体外循环时肺泡灌洗液中ＳＰＡ含量，与术前比较有明显下降，结果与临床符合，本文所建立的方法是一种敏感度高，简单实用研究肺表面活性物质的有效

The determination of surfactant protein A with Western-dot-blot analysis and its clinical application

The determination of the concentration of surfactant protein A(SPA) could serve as an index of fetal lung maturity, adjunct to the diagnosis of its lung disorders, follow-up of the therapeutic efficacy and an index of prognostic evaluation. Western-dot-blot analysis method was established for the determination of SPA. The amniotic fluid concentration of SPA in 55 pregnant women of 36 - 42 gestational weeks were determind. The results showed that the concentration of SPA elevated with the increase of the gestational weeks. The concentration of SPA in the pulmonary lavage fluid in 26 pediatric patients under thoracic surgery were also determined before the operation and during the extracorporal circulation. The concentration of SPA in the lavage nuid was found significantly lower than that before the operation. The method established is a highly sensitive, simple and practical, and being an effective tool for studying the surfactant protein.