快中子诱导人鼻咽癌细胞系凋亡的研究

目的　研究中子不同剂量照射人鼻咽癌（ＣＮＥ２） 细胞凋亡发生特点及与Ｘ射线所致凋亡的差异。探讨凋亡在中子治疗肿瘤中的作用及临床意义。方法　采用琼脂糖凝胶电泳及ＤＮＡ特异性荧光染色方法（Ｈｏｅｃｈｓｔ３３３４２） 检测照射后不同时间人鼻咽癌（ＣＮＥ２）细胞。结果　发现中子可诱导人鼻咽癌细胞发生凋亡，这种凋亡发生存在着一定的时间剂量相关性。在相同剂量照射下，同一时间点上，中子照射所致的凋亡反应强于Ｘ 射线所致的凋亡。结论　快中子照射离体细胞可引起较强的凋亡反应。中子杀伤肿瘤的机理可能也是主要通过凋亡途径来实现的。

Apoptosis of nasopharyngeal carcinoma cell line(CNE-2) induced by neutron irradiation

Objective To study the apoptotic response of the nasopharyngeal carcinoma cell line (CNE 2) induced by neutron irradiation. Methods CNE 2 cells were cultured as usual.Using the techniques of DNA agarose gel electrophoresis and DNA special fluorescent staining , the status of apoptosis in CNE 2 cells after neutron irradiation was detected. Results It was shown that the apoptosis can be induced in CNE 2 cell after neutron radiation.Six hrs,after different doses of neutron(0/0.667/1.333/2.000/ 2.667 / 3.333Gy) and X ray (0/2/4/6/8/10 Gy) irradiation the apoptotic rates were 2 4%,6 3%,7 1%,9 5%,13 5%,14 6% and 2 4%,3 8%,5 7%,7 8%,10 4%,11 7%, respectively;at 48hrs they were 18 3%,21 5%,22 8%,29 3%,34 2% and 13 7%,17 6%,21 3%,25 6%,28 9%, respectively.At 10hrs after neutron irradiation the DNA ladder of apoptosis could be detected between 0 667- 3 333 Gy doses in CNE 2 cells by DNA agarose gel electrophoresis. Conclusion Neutron radiation can induceapoptosis in tumor cells.Compared with the X ray,neutron induces apoptosis in larger extent than X ray in the same condition;meanwhile,apoptosis after irradiation is dose and time dependent.