DNA凝胶电泳和放射自显影研究153Sm诱发骨肉瘤细胞凋亡

目的　研究受１５３ＳｍＥＤＴＭＰ内照射治疗时诱发骨肉瘤细胞凋亡的ＤＮＡ损伤特征，以及１５３Ｓｍ 核素在瘤细胞中的行径特性。方法　观察了１５３ＳｍＥＤＴＭＰ内照射不同时间，诱发骨肉瘤细胞凋亡的ＤＮＡ 凝胶电泳和微观放射自显影的示踪动态。结果　研究发现，在１５３ＳｍＥＤＴＭＰ内照射作用下，骨肉瘤细胞的ＤＮＡ 呈现出在核小体间断裂的阶梯状条带形成的凋亡特征，放射性核素１５３Ｓｍ 可迅速透过瘤细胞膜，也可被瘤细胞吞噬，呈亲膜性积聚和膜包裹着的凋亡小体呈现。结论　１５３ＳｍＥＤＴＭＰ内照射治疗时，可诱发骨肉瘤细胞凋亡发生，其诱发凋亡的程度随１５３Ｓｍ 内照射作用时间延长而增升。

DNA gel electrophoretic and microautoradiographic studies on apoptosis in bone tumor cells after exposure to 153 Sm|EDTMP

Objective To study the characteristics of injury in apoptotic bone tumor cells and behavior of radionuclide {} {153}Sm in tumor cells. Methods DNA gel electrophoresis and microautoradiographic tracing of apoptotic bone tumor cells at different intervals after {} {153}Sm|EDTMP internal irradiation. Results Bone tumor cells internally irradiated with 153 Sm|EDTMP displayed characteristics of DNA ladder formation in apoptotic cells. 153 Sm could permeate through tumor cell membrane and be phagocytized by the tumor cells,showing membrane|seeking and membrane bounded apoptotic bodies condensation. Conclusion Progression of apoptosis in bone tumor cells induced by 153 Sm|EDTMP is dependent on the time elapse of 153 Sm internal irradiation.;