rhTPO/GM-CSF融合蛋白对60Co γ射线照射小鼠造血功能的影响

目的 探索一个新的、具有能同时促进机体红、粒及巨核细胞系造血的细胞因子，以便治疗由化疗和放疗等各种原因引起全血细胞损伤的综合征。方法 所用的细胞因子是本实验室重组的产品，以照射小鼠为模型，给小鼠注射细胞因子后，观察红、粒及巨核细胞的变化。结果 ｒｈＴＰＯ／ＧＭ－ＣＳＦ融合蛋白对受照射小鼠血象有恢复作用。结论 融合蛋白具有ＴＰＯ－ＣＳＦ双重的生物活性。

Influence of rhTPO/GMCSF fusion protein on hemopoiesis in mice irradiated with 60 Co rays

Objective To find a new biological therapy for secondary hematopoietic failure including anemia, infection and hemorrhage after administration of chemotherapeutic drugs etc. Methods hGM-CSF gene was ligated with hTPO gene isolated from human fetal liver mRNA and a new fusion protein rhTPO/GMCSF obtained. Results The new fusion protein could promote recovery of peripheral WBC and PLT of 5.0 Gy irradiated mice.BFU-E, CF-UMeg and CF-UGM in bone marrow of mice after irradiation recovered significantly by treatment with rhTPO/GM-CSF fusion protein for 10 days. Conclusion These results suggest that the new fusion protein has the biological activity of both hTPO and hGM-CSF simultaneously and can stimulate the proliferation of megakaryocyte and granulocyte progenitors.