In vivo and in vitro effects of 1,1-dimethylhydrazine on selected immune functions |
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| Authors: | M J Tarr R G Olsen D L Jacobs |
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| Affiliation: | 1. Faculty of Geography, Lomonosov Moscow State University (MSU), Moscow, Russian Federation;2. Sector of Molecular Evolution, Institute for Information Transmission Problems (Kharkevich Institute) of the Russian Academy of Sciences, Moscow, Russian Federation;1. Xi’an Research Institute of High Technology, Xian 710025, China;2. School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Science, Taian 271000, China;1. High-Tech Institute of Xi’an, Shaanxi 710025, China;2. School of Chemical and Pharmacological Engineering, Huanghuai University, Zhumadian, Henan 463000, China;3. High-Tech Institute of Beijing, 10085, China;1. Department of Anesthesiology, Soroka University Medical Centerand the Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel;2. General Intensive Care Unit, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel;3. Department of Family Medicine, Clalit Health Services andFaculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel;1. Institute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice CZ-532 10, Czech Republic;2. Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice CZ-532 10, Czech Republic;3. Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice CZ-532 10, Czech Republic |
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| Abstract: | ![]()
The in vivo phase of the experiments reported here include the evaluation of immune function after short-or long-term treatment of mice with 1,1-dimethylhydrazine (UDMH). Long-term exposure (3 injections/week for 14 weeks) resulted in increased numbers of Jerne plaque-forming cells, a trend toward decreased induction of suppressor cell activity by concanavalin A (Con A), and no effects on mitogen-induced lymphocyte blast transformation (LBT), compared to saline-treated control mice. These effects were greatest at doses of 10 or 50 mg/kg, while higher doses had less of an effect. In vitro experiments were performed by adding UDMH to normal murine splenocytes in the LBT assay and con A-induced suppressor cell assay. The UDMH induced a significant enhanced response to lipopolysaccharide (LPS) at 10 and 50 micrograms/ml, and a suppressed response to both Con A and LPS at higher concentrations. The UDMH also caused a decrease in suppressor cell activity at 25 micrograms/ml. Selective abrogation of suppressor activity or alteration of the suppressor cell-helper ratio were suggested as possible mechanisms for the enhancement effect associated with UDMH. |
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