高原肺水肿的血浆蛋白质组学研究

目的 探讨高原肺水肿患者血浆蛋白组中差异表达的蛋白质及其意义.方法 抽取拉萨市(海拔3658m)6例高原肺水肿患者和6例健康对照者的血浆,去除血浆高丰度蛋白,采用2-DE分离血浆/血清低丰度蛋白质,酶解差异表达的蛋白质斑点,采用MALDI-TOF/MS检测肽指纹谱,并在GenBank数据库中进行比对分析.结果 与对照组比较,高原肺水肿患者免疫球蛋白k1轻链、血清转铁蛋白前体、α-胰蛋白酶抑制剂重链相关蛋白表达上调,人纤维胶凝蛋白3表达下调.结论 高原肺水肿患者血浆中上述4种蛋白的差异表达可能与高原肺水肿的发生有关,有可能成为预测该疾病的新靶点.

Study on plasma proteomics in patients with high altitude pulmonary edema (HAPE)

Objective To investigate the differential expressions of protein in the plasma proteome in patients suffering from high altitude pulmonary edema(HAPE) and their implications.Methods The plasma of six HAPE patients and six healthy controls was studied.The high-abundant proteins in plasma were removed.The low-abundant proteins in the plasma/serum were segregated by 2-DE.MALDI-TOF/MS was adopted to measure the peptide dactylogram after the differential proteins spots were digested by enzymes.Comparison and analysis were made in the GeneBank.Results The immunoglobulin K1 light chain,serum transferrin protein precursor,and α-trypsin inhibitor heavy chain-related protein expression were increased in HAPE patients compared with those of the control group.However the human fibrin glue coagulation protein 3 was down-regulated.Conclusion The differential expression of the above four proteins in the plasma of HAPE patients may be related to the occurrence of HAPE,and can be used as the target spots for the prediction of HAPE.