| 参麦注射液细菌内毒素检测方法的研究 |
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| 引用本文： | 陈华龙,王莉蓓,姜丽君.参麦注射液细菌内毒素检测方法的研究[J].海峡药学,2010,22(10):54-55. |
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| 作者姓名： | 陈华龙 王莉蓓 姜丽君 |
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| 作者单位： | 广东韶关市药品检验所,韶关,512028 |
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| 摘 要： | 目的应用动态浊度法定量检测参麦注射液中的细菌内毒素。方法采用《中国药典》2005年版二部附录细菌内毒素定量检测法。结果将参麦注射液进行1∶20稀释,可排除干扰,回收率在50%-200%,无干扰作用。结论应用动态浊度法测定参麦注射液中的细菌内毒素是可行的,此方法既可避免家兔热原检查法操作复杂的缺点,同时又避免了限量法检测内毒素的干扰。
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| 关 键 词： | 动态浊度法 参麦注射液 细菌内毒素 干扰实验 |
Bacterial endotoxins test for Shenmai injection by dynamic turbidimetric assay |
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| Institution: | CHEN Hua-long(Shaoguan Institute for Drug Control,Guangdong 512028,China) |
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| Abstract: | OBJECTIVE To establish a determination of endotoxin in Shenmai injection by dynamic turbidimetric assay.METHODS Inhibition or enhancement test for bacterial endotoxins test were used according to Chp 2005.RESULTS 1∶20 solution of Shenmai Injection showed no interference on test and the recovery was 50%～200%.CONCLUSION Bacterial endotoxin test for Shenmai injection by turbidimetric assay has been established and could be used as an altermative for the rabbit pyrogen test. |
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| Keywords: | Turbidimetric assay Shenmai injection Bacterial endotoxin interfering test |
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