巢式PCR检测胎儿组织人细小病毒B19感染

作用设计合成的两对引物建立了巢式ＰＣＲ－ＥＢ染色法检测Ｂ１９ＤＮＡ新技术，扩增产物为１０４ｂｐ片段，敏感性达０．００７ｆｇ，是一般ＰＣＲ的１００倍，是一种特异、敏感、简便、快速诊断Ｂ１９感染的新方法。用该方法检测了５７例自然流产和死胎的胎儿组织，１７例阳性，其中自然流产１１／４２（２６５），死胎６／１５（４０％），证实了国内胎儿有Ｂ１９病毒感染，和自然流产、死胎可能相关，并对ＰＣＲ实验中防污染措

Detection of human parvovirus B19 infections in fetal tissues by nest-ed PCR

A new nested PCR-EB staining assay technique to detect B19 DNA was established by using two pairs of primers system. The amplified Products are 104 bp DNA fragments and its sensitivity is 0.007 fg,100 times more sensitive than the single PCR. This method is specific,sensitive,simple and rapid in diagnos- ing B19 infections.By this method,57 fetal tissues of spontaneous abortions and still births were detected. Among which 17 were positive.In these positive samples,spontaneous abortions were 11/42(26%)and still births were 6/15(40%).These results verify that there are B19 infections in fetus in China and that there may be possible relativity among spontaneous abortion,still birth and B19 infection. Furthermore, measures of avoiding contamination in PCR tests were also discussed in this study.