| 孑遗植物杜仲的遗传多样性RAPD分析和保护策略研究 |
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| 引用本文： | 王瑷琦,黄璐琦,邵爱娟,崔光红,陈敏,佟长辉.孑遗植物杜仲的遗传多样性RAPD分析和保护策略研究[J].中国中药杂志,2006,31(19):1583-1586. |
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| 作者姓名： | 王瑷琦 黄璐琦 邵爱娟 崔光红 陈敏 佟长辉 |
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| 作者单位： | 1. 中国中医科学院,中药研究所,北京,100700
2. 吉林省公安厅,安康医院,吉林,公主岭,136101 |
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| 基金项目： | 科技部社会公益研究项目 |
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| 摘 要： | 目的:进行杜仲群体遗传多样性的研究。方法:采用随机扩增多态DNA(RAPD)方法对杜仲的16个群体、260个个体进行遗传多样性分析。结果:用10个随机引物共扩出110条清晰条带,其中多态性条带为106条,总的多态位点百分率为96.36,平均多态位点百分率为38.92。Ne’is基因多样性指数H=0.246 1,Shannon’s多态性信息指数I=0.386 8。基因分化系数Gst=0.424 4。结论:杜仲种内具有丰富的遗传多样性,而不同群体内部的遗传多样性低。遗传分化分析表明杜仲群体间已发生了高度的遗传分化,因此,生物多样性保护时应尽可能保护更多的种群。
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| 关 键 词： | 杜仲 遗传多样性 保护 |
| 文章编号： | 1001-5302（2006）19-1583-04 |
| 收稿时间： | 2005-12-01 |
| 修稿时间： | 2005-12-01 |
Genetic diversity of Eucommia ulmoides by RAPD analysis |
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| WANG Ai-qi,HUANG Lu-qi,SHAO Ai-juan,CUI Guang-hong,CHEN Min,TONG Chang-hui.Genetic diversity of Eucommia ulmoides by RAPD analysis[J].China Journal of Chinese Materia Medica,2006,31(19):1583-1586. |
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| Authors: | WANG Ai-qi HUANG Lu-qi SHAO Ai-juan CUI Guang-hong CHEN Min TONG Chang-hui |
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| Institution: | 1. Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China; 2. Ankang Hospital, Jilin Public Security Department, C, ongzhuling 136101, China |
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| Abstract: | Objective: To determine the genetic diversity of Eucommia ulmoides.Method: 260 samples of 20 populations were analyzed through radom amplified polymorphic DHA(RAPD).Result: Total polymorphic loci percentage was 96.36 and the average was 38.92.110 bands were produced with 10 random primers and 106 were polymorphic.Nei's gene diversity(H)was 0.246 1,Shannon's Information index(I)was 0.386 8,Gst was 0.424 4,indicating that 42.44% of the genetic variation was distributed among populations and 57.65% within populations.Conclusion: The genetic variation was relatively high in E.ulmoides,so the genetic diversity conservation principle should mainly focus on protection of the populations. |
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| Keywords: | RAPD |
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