| 原发性肝癌Twist基因表达的研究 |
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| 引用本文： | 徐静,陈孝平,向秋. 原发性肝癌Twist基因表达的研究[J]. 中华肿瘤防治杂志, 2007, 14(13): 994-996 |
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| 作者姓名： | 徐静 陈孝平 向秋 |
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| 作者单位： | 广西医科大学第一附属医院外科,广西,南宁,530007;华中科技大学同济医学院肝胆胰外科研究所,湖北,武汉,430030;桂林医学院生命科学中心,广西,桂林,541001 |
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| 基金项目： | 广西地方性高发疾病防治研究重点实验室基金 |
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| 摘 要： | 目的：研究Twist基因在人原发性肝癌（primary liver cancer,PLC）组织中的表达及其临床意义。方法：应用免疫组织化学SABC染色方法检测45例PLC组织及癌旁组织中Twist蛋白并采用RT-PCR对TwistmR-NA的表达进行分析研究。结果：PLC组织、癌旁组织及正常肝组织中Twist的阳性表达率分别为80.0%、57.8%和22.2%。PLC和癌旁组织与正常对照肝组织中Twist的表达相比较差异均有统计学意义,P〈0.05。肿瘤包膜也可见Twist表达。RT-PCR检测Twist结果显示,PLC组织中Twist mRNA表达显著上调。结论：PLC组织中高表达转录因子Twist对肿瘤的发生、发展起着重要作用。
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| 关 键 词： | 肝肿瘤/病理学 Twist 基因表达 免疫组织化学 |
| 文章编号： | 1673-5269（2007）13-0994-03 |
| 收稿时间： | 2006-08-09 |
| 修稿时间： | 2006-08-092006-12-11 |
Study on expression of Twist gene in primary liver cancer |
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| XU Jing,CHEN Xiao-ping,XIANG Qiu. Study on expression of Twist gene in primary liver cancer[J]. Chinese Journal of Cancer Prevention and Treatment, 2007, 14(13): 994-996 |
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| Authors: | XU Jing CHEN Xiao-ping XIANG Qiu |
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| Affiliation: | 1. Department of Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530007, P. R. China ;2. Institute of Hepato-biliary Pancreatic surgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, P. R. China ;3. Bio-technology Center of Guilin Medical College, Guilin 541001, P. R. China |
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| Abstract: | OBJECTIVE:To demonstrate the possibility of overexpression of Twist in primary liver cancer (PLC). METHODS: Twist expression was investigated by the immunohistochemical analysis and RT-PCR assay in 45 patients with PLC and the control tissues which were obtained from 9 patients with liver hemangioma. RESULTS: The positive rates of Twist expression were 80.0%, 57.8% and 22.2% in the PLC tissues, noncancerons adjacent areas and control tissues, respectively. It was found that there was Twist expression in tumor membrane. The result of RT-PCR assay revealed that the expression of Twist was stronger in the cancerous tissues than those in the noncancerous adjacent and normal liver tissues. CONCLUSION: The expression of Twist is up-regulated in PLC, and it plays an important role in progression of tumor. |
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| Keywords: | Twist |
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