| 粉防己碱对大鼠心肌细胞电压依赖性钙通道的作用 |
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| 引用本文： | 陈金明,钟纪根.粉防己碱对大鼠心肌细胞电压依赖性钙通道的作用[J].中国药理学通报,1996,12(3):226-228. |
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| 作者姓名： | 陈金明 钟纪根 |
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| 作者单位： | 第二军医大学长征医院心内科，第二军医大学病理生理学教研室 |
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| 摘 要： | 运用钙离子荧光指示剂Ｆｕｒａ－２／ＡＭ，检测了粉防己碱（Ｔｅｔ）对成年大鼠心室肌细胞电压依赖性钙通道的影响。结果显示：基础状态下心肌细胞内钙离子（［Ｃａ２＋］ｉ）为１６２．６±７．３ｎｍｏｌ·Ｌ－１，５０ｍｍｏｌ·Ｌ－１氯化钾能使［Ｃａ２＋］ｉ增加至４８０．８±９．３ｎｍｏｌ·Ｌ－１（Ｐ＜０．０１），在无细胞外钙条件下，这种增加作用消失，而预先给予Ｔｅｔ和维拉帕米（Ｖｅｒ）则能阻断高钾升高［Ｃａ２＋］ｉ的作用。结果提示：Ｔｅｔ是通过阻断电压依赖性钙通道而发挥作用的。
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| 关 键 词： | Fura－2 钙离子 粉防己碱 维拉帕米 心肌细胞 大鼠 |
The effect of tetrandrine on the voltage-dependent calcium channel of myocardial cells in rats |
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| CHEN Jin-Ming,CHEN Si-Cong,ZHONG Ji-Gen,ZHANG Guo-Yuan,GONG Xiao-Qi, WU Zhong-Gui.The effect of tetrandrine on the voltage-dependent calcium channel of myocardial cells in rats[J].Chinese Pharmacological Bulletin,1996,12(3):226-228. |
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| Authors: | CHEN Jin-Ming CHEN Si-Cong ZHONG Ji-Gen ZHANG Guo-Yuan GONG Xiao-Qi WU Zhong-Gui |
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| Abstract: | The effect of tetrandrine on voltage-dependent calcium channel of ventricular myocyte was investigated by fluorescein Fura-2/AM in rats.It was showed that the concentration Of Ventricular myocyte Ca2+]i WaS 162. 6±7.3nmol·L-1.50mmol·L 1 KCl could increase signiflcantly Ca2+]i(480.6±9.3nmol·L-1 ,P<0.01),Pretreatment of tetrandrine and verapamil could block KCl-induced increase of Ca2+] i.Under the condition of extracellular calcium free,the effect of KCl-induced increase Ca2+] i disappeared.The results Suggest that like verapamil, tetrandrine plays it' s pharmacological effects via blocking voltage-dependent calcium channel. |
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| Keywords: | Fura-2 calcium tetrandrine verapamil myocardial cells rats |
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