土槿皮乙酸对大鼠脑胶质瘤C6细胞增殖的抑制作用

目的 研究土槿皮乙酸(pseudolaric acid B,PAB)对大鼠脑胶质瘤C6细胞增殖的抑制作用.方法 采用MTT法检测0、0.1、0.2、0.4、0.8、1.6、8、16 μg/ml PAB对C6细胞增殖能力的影响;流式细胞术检测碘化丙啶(PI)染色后细胞周期的变化;皮下注射C6细胞建立C6种植瘤大鼠模型并测量种植瘤体积;免疫组化法测定大鼠脑胶质瘤C6细胞种植瘤组织中增殖细胞核抗原(PCNA)的表达变化.结果 体外实验结果表明,PAB呈剂量依赖性地抑制胶质瘤C6细胞生长,半数致死剂量(IC50)为0.03 μg/ml,同时,可使C6细胞周期阻滞于G2/M期;体内实验结果表明,PAB给药后大鼠种植瘤体积有所降低,种植瘤组织中PCNA表达呈下降趋势.结论 PAB可抑制大鼠脑胶质瘤C6细胞增殖,阻滞细胞周期中G2/M期、下调PCNA表达为其可能的作用机制.

Inhibitory effect of pseudolaric acid B on proliferation of rat glioma C6 cells

Objective To observe the inhibitory effects of pseudolaric acid B(PAB) on the proliferation of rat glioma C6 cells in vivo and in vitro.Methods MTT assay was used to examine the inhibitory effect of 0,0.1,0.2,0.4,0.8,1.6,8,and 16 μg/ml PAB on C6 cells;the distribution of cell cycle was determined by flow cytometry.C6 cells were injected subcutaneously to establish glioma-bearing rat model and the tumor volume was measured.The expression of proliferating cell nuclear antigen(PCNA) was detected immunohistochemically in the implanted glioma.Results In vitro results showed that PAB inhibited C6 cell growth in a dose-dependent manner,with the IC50 being 0.03 μg/ml.It also arrested the cells in G2/M phase.In vivo results showed that the tumor size of PAB group was decreased compared with the control group;moreover,PCNA expression was also decreased by PAB.Conclusion PAB can inhibit rat glioma C6 cell growth,which might be associated with cell arrest in G2/M phase and down-regulation of PCNA expression.