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| 川楝素对K562细胞增殖和凋亡作用的影响 | |
| 引用本文： | 王进,刘小玲,王鹏,蒋冬韵,何於娟. 川楝素对K562细胞增殖和凋亡作用的影响[J]. 医学争鸣, 2009, 30(22): 2528-2532 |
| 作者姓名： | 王进 刘小玲 王鹏 蒋冬韵 何於娟 |
| 作者单位： | 重庆医科大学临床检验诊断学教育部重点实验室,重庆,400016;北京大学医学部2005级基础医学专业,北京,100083 |
| 基金项目： | 重庆市科委自然科学基金(CSTC,2009BB5258); 重庆医科大学校级课题(0200101162) |
| 摘 要： | 目的：探讨川楝素对人白血病K562细胞增殖和凋亡作用的影响.方法：MTT法检测川楝素对K562细胞和健康成人外周血单个核细胞（peripheral blood mononuclear cell,PBMC）的增殖抑制率.瑞氏染色观察川楝素对K562细胞、PBMC形态的影响.透射电镜观察K562细胞超微结构改变.流式细胞术检测细胞凋亡率.琼脂糖凝胶电泳法观察凋亡细胞DNA片段化现象.比色法检测Caspase-3,Caspase-8,Caspase-9活性变化.免疫组化法检测凋亡相关蛋白Bcl-xl,Bax和Fas的表达.结果：川楝素对K562细胞有明显增殖抑制作用,呈时间和浓度依赖性（P〈0.01）,作用72h后IC50值为（52.13±0.82）nmol/L,而对PBMC几乎无影响.普通光镜下分别观察K562细胞和PBMC,K562细胞可见典型的凋亡形态学改变,PBMC形态无明显变化.超微结构显示K562细胞核染色质聚集,固缩,可见凋亡小体;以10,30,50nmol/L川楝素处理细胞72h后,K562细胞早期凋亡率分别为9.66%,26.06%,52.70%（P〈0.01）;琼脂糖凝胶电泳可见典型“梯状”条带;川楝素激活K562细胞Caspase-3,Caspase-8,Caspase-9活性;凋亡相关蛋白Bcl-xl表达降低,Bax,Fas表达增强（P〈0.05）.结论：川楝素对K562细胞与PBMC之间具有选择抑制作用和诱导K562细胞凋亡的作用,可能通过线粒体途径和死亡受体途径双重机制介导. |
| 关 键 词： | 川楝素 K562细胞 增殖抑制 凋亡 |
Effect of toosendanin on proliferation and apoptosis of K562 cells |
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| WANG Jin,LIU Xiao-Ling,WANG Peng,JIANG Dong-Yun,HE Yu-Juan Key Laboratory of Clinical Laboratory Diagnostics,Ministry of Education,Chongqing Medical University,Chongqing ,China,Basic Medical School,Peking University,Beijing. Effect of toosendanin on proliferation and apoptosis of K562 cells[J]. Negative, 2009, 30(22): 2528-2532 | |
| Authors: | WANG Jin LIU Xiao-Ling WANG Peng JIANG Dong-Yun HE Yu-Juan Key Laboratory of Clinical Laboratory Diagnostics Ministry of Education Chongqing Medical University Chongqing China Basic Medical School Peking University Beijing |
| Affiliation: | WANG Jin1,LIU Xiao-Ling1,WANG Peng1,JIANG Dong-Yun2,HE Yu-Juan1 1Key Laboratory of Clinical Laboratory Diagnostics,Ministry of Education,Chongqing Medical University,Chongqing 400016,China,2Basic Medical School,Peking University,Beijing 100083 |
| Abstract: | AIM:To study the effects of proliferation and apoptosis on K562 cells induced by toosendanin.METHODS:The growth inhibition rate of K562 cells and Peripheral Blood Mononuclear Cell(PBMC)was measured by MTT.Morphology of K562 cells and PBMC was detected by wright's stain.The ultrastructure changes of K562 cells were analyzed by transmission electron microscope.DNA fragmentation and the percentage of apoptoticcells were examined by Sepharose electrophoresis and flow cytometry(FCM),respectively.The activities o... |
| Keywords: | toosendanin K562 cell proliferation inhibition apoptosis |
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