| 8-烷基小檗碱同系物对蛋白荧光的淬灭作用与其抗菌活性的关系 |
| |
| 引用本文： | 杨勇,张保顺,叶小利,张华珊,李学刚. 8-烷基小檗碱同系物对蛋白荧光的淬灭作用与其抗菌活性的关系[J]. 医学争鸣, 2009, 30(21): 2363-2365 |
| |
| 作者姓名： | 杨勇 张保顺 叶小利 张华珊 李学刚 |
| |
| 作者单位： | 怀化医学高等专科学校药学系天然药物化学教研室,湖南,怀化,418000;西南大学药学院药用资源化学研究所,重庆,400716;西南大学生命科学学院生化教研室,重庆,400716 |
| |
| 摘 要： | 目的：了解8-烷基小檗碱同系物（Ber-C8-n）对蛋白荧光的淬灭作用与其抗菌活性的关系,探讨Ber-C8-n抗菌活性变化的机制.方法：以牛血清白蛋白（BSA）为实验材料,等渗磷酸缓冲液（PBS）为溶剂,分别将每种Ber-C8-n用PBS溶液配制不同浓度的梯度溶液,测定BSA与不同浓度Ber-C8-n在PBS体系中反应后苯丙氨酸（Phe）,色氨酸（Trp）和酪氨酸（Tyr）残基的荧光强度,观察Ber-C8-n对BSA残基荧光的淬灭情况.结果：Ber-C8-n对BSA中3种氨基酸残基的荧光均有不同程度的淬灭作用,并均有明显的剂量效应.比较后发现,Ber-C8-n对BSA中Phe,Trp和Tyr残基荧光的淬灭作用表现出相似规律,即Ber-C8-8同样表现了最强的荧光淬灭作用,其次为烷基链较长或较短的Ber-C8-n,基本上呈现以Ber-C8-8顶点的抛物线.3种残基之间比较,Ber-C8-8对Trp残基荧光的淬灭作用明显强于对Tyr和Phe残基的作用.结论：抗菌活性越强的Ber-C8-n对BSA荧光的淬灭作用也越强,Ber-C8-n抗菌活性的差异可能是不同Ber-C8-n与目标蛋白的富Trp残基区的结合能力差异导致的．
|
| 关 键 词： | 8-烷基小檗碱同系物 抗菌机制 荧光淬灭 牛血清白蛋白 |
Relationship of fluorescence quenching effect of 8-alkyl-berberine homologues on bovine serum albumins and their antimicrobial activities |
| |
| YANG Yong,ZHANG Bao-Shun,YE Xiao-Li,ZHANG Hua-Shan,LI Xue-GangNatural Medicine Chemistry Staff Room. Relationship of fluorescence quenching effect of 8-alkyl-berberine homologues on bovine serum albumins and their antimicrobial activities[J]. Negative, 2009, 30(21): 2363-2365 |
| |
| Authors: | YANG Yong ZHANG Bao-Shun YE Xiao-Li ZHANG Hua-Shan LI Xue-GangNatural Medicine Chemistry Staff Room |
| |
| Affiliation: | YANG Yong1,ZHANG Bao-Shun2,YE Xiao-Li3,ZHANG Hua-Shan3,LI Xue-Gang21Natural Medicine Chemistry Staff Room,Department of Pharmaceutical Science,Huaihua Medical College,Huaihua 418000,China,2Chemistry Institute of Pharmaceutical Resources,School of Pharmaceutical Science,3Biochemistry Staff Room,College of Life Science,Southwest University,Chongqing 400716 |
| |
| Abstract: | AIM:To investigate the relationship of antimicrobial activity and fluorescence quenching effect of 8-alkyl-berberine homologues(Ber-C8-n)including berberine,8-ethyl-berberine,8-butyl-berberine,8-hexyl-berberine,8-octyl-berberine,8-decyl-berberine,8-dodecyl-berberine and 8-cetyl-berberine on protein and the changing mechanism of antimicrobial activity. METHODS:With bovine serum albumins(BSA) as experimental material and phosphate buffered saline(PBS) as reacting solvent,the fluorescence intensity of Tryptoph... |
| |
| Keywords: | 8-alkyl-berberine homologues antimicrobial mechanism fluorescence quenching effect bovine serum albumins |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
