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| 自制抗体的颗粒增强透射免疫分析法测定人血清胱抑素C的评价 | |
| 引用本文： | 陈婷梅,俸家富,郭变琴,文阳安,涂植光. 自制抗体的颗粒增强透射免疫分析法测定人血清胱抑素C的评价[J]. 医学争鸣, 2009, 30(21): 2299-2301 |
| 作者姓名： | 陈婷梅 俸家富 郭变琴 文阳安 涂植光 |
| 作者单位： | 重庆医科大学检验系,教育部临床检验诊断学重点实验室,重庆,400016;绵阳市中心医院检验科,四川,绵阳,621000 |
| 摘 要： | 目的：用自制抗体研制可在自动生化分析仪应用的颗粒增强透射免疫浊度分析法（PETIA）定量测定人血清胱抑素C（Cys C）的试剂盒.方法：构建人CysC的原核表达载体pET32a（＋）/Cys C,纯化Cys C重组蛋白并免疫大白兔,制备Cys C抗血清;以自制的CysC抗血清包被乳胶颗粒,研制PE-TIA法定量检测血清Cys C的试剂盒,并对其分析性能进行方法学评价.结果：本法回收率为97.5%～105.0%,批内及批间CV均〈5%,线性范围8.11～0.25mg/L,检测限0.2mg/L;本法与Dako法和BN-Prospect法呈正相关（P〈0.0001）;胆红素对高浓度Cys C测定有干扰作用,Hb和葡萄糖对本法无干扰.结论：应用自动生化分析仪的自研法性能满意. |
| 关 键 词： | 胱抑素C 抗血清 颗粒增强透射免疫浊度分析法 方法学评价 |
Evaluation of particle enhanced turbidimetric immunoassay method for human serum Cys C with self-preparing antibody |
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| CHEN Ting-Mei,FENG Jia-Fu,GUO Bian-Qin,WEN Yang-An,TU Zhi-GuangKey Laboratory of Clinical Diagnosis of Education Ministry,Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing ,China. Evaluation of particle enhanced turbidimetric immunoassay method for human serum Cys C with self-preparing antibody[J]. Negative, 2009, 30(21): 2299-2301 | |
| Authors: | CHEN Ting-Mei FENG Jia-Fu GUO Bian-Qin WEN Yang-An TU Zhi-GuangKey Laboratory of Clinical Diagnosis of Education Ministry Faculty of Laboratory Medicine Chongqing Medical University Chongqing China |
| Affiliation: | CHEN Ting-Mei1,FENG Jia-Fu2,GUO Bian-Qin1,WEN Yang-An1,TU Zhi-Guang11Key Laboratory of Clinical Diagnosis of Education Ministry,Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,China,2Department of Laboratory,Mianyang Central Hospital,Mianyang 621000 |
| Abstract: | AIM:To develop a kit of particle enhanced turbidimetric immunoassay (PETIA) method based on self-made antibody for determining human serum Cys C level with automatic biochemical analyzer. METHODS:The prokaryotic expression vector pET32a(+)/Cys C was constructed,in which Cys C expression was induced. The antiserum was prepared after rabbits were immunized with the purified recombinant protein. Then the antibody was covalently attached to the uniform microparticles,a kit of PETIA method for human serum Cys C ... |
| Keywords: | cystatin C antiserum particle enhanced turbidimetric immunoassay evaluation test of methodology |
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