| 细胞连接调控蛋白在大鼠胰腺癌和非癌胰腺组织中表达及意义 |
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| 引用本文： | 邹琼,杨竹林,刘洁琼,杨乐平,苗雄鹰,李龙清.细胞连接调控蛋白在大鼠胰腺癌和非癌胰腺组织中表达及意义[J].第四军医大学学报,2009(22):2595-2598. |
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| 作者姓名： | 邹琼 杨竹林 刘洁琼 杨乐平 苗雄鹰 李龙清 |
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| 作者单位： | 中南大学湘雅三医院病理科;中南大学湘雅二医院肝胆胰疾病研究室; |
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| 摘 要： | 目的：建立SD大鼠胰腺癌模型,研究细胞连接调控蛋白（Claudin-1,Occludin,E-cad,Snail）在胰腺癌发生发展过程中的作用及其意义.方法：90只大鼠随机分为二甲基苯荓蒽（DMBA）组（A组,n=40）,DMBA＋曲古霉素A（TSA）组（B组,n=40）和空白对照组（C组,n=10只）.将DMBA直接置入A,B组胰腺实质内建立胰腺癌模型,B组腹腔注射TSA,～5mo内处死并观察胰腺癌发生情况,C组于第5mo处死.采用En-VisionTM免疫组化法检测Claudin-1,Occludin,E-cad,Snail的表达.结果：A组3～5mo癌发生率为48.6%（18/37）,5mo（60.0%）高于4mo组（40.0%）和3mo组（28.6%）.B组3～5mo癌发生率为33.3%（12/36）,5mo（40.0%）高于4mo组（30.0%）和3mo组（16.7%）.A组发癌率高于B组.A组,B组胰腺导管癌分别为17例和11例,其余为纤维肉瘤;A组胰腺癌最大径均值大于B组（A组：0.5～1.0cm7例,1.0～2.0cm10例,〉2.0cm1例;B组：0.5～1.0cm9例,1.0～2.0cm2例,〉2.0cm1例,P〈0.05）;C组胰腺和A,B,C组胰腺外主要脏器均未见明显病理改变.A组＋B组胰腺导管癌Clau-din-1,Occludin,E-cad表达阳性率明显低于A组＋B组非癌胰腺组织（P〈0.05或P〈0.01）,但Snail则相反（P〈0.01）;在胰腺导管腺癌中Claudin-1,Occludin,E-cad表达均相互呈一致性（P〈0.05或P〈0.01）,但三者均与Snail表达呈明显不一致性（P〈0.05或P〈0.01）.结论：较大剂量DMBA置入胰实质内可在短期内获得较高的SD鼠胰腺癌发生率,TSA能抑制胰腺癌发生和生长;Claudin-1,Occludin,E-cad和Snail在DM-BA诱导SD大鼠胰腺癌发生中可能有重要作用.
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| 关 键 词： | 胰腺 肿瘤 动物模型 Claudins Occludin 钙粘附蛋白 Snail |
Expression of regulated proteins of cell junctions and their significance on the carcinogenesis of Sprague-Dawely (SD) rat pancreas |
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| ZOU Qiong,YANG Zhu-Lin,LIU Jie-Qiong,YANG Le-Ping,MIAO Xiong-Ying,LI Long-Qing.Expression of regulated proteins of cell junctions and their significance on the carcinogenesis of Sprague-Dawely (SD) rat pancreas[J].Journal of the Fourth Military Medical University,2009(22):2595-2598. |
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| Authors: | ZOU Qiong YANG Zhu-Lin LIU Jie-Qiong YANG Le-Ping MIAO Xiong-Ying LI Long-Qing |
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| Institution: | ZOU Qiong1,YANG Zhu-Lin2,LIU Jie-Qiong2,YANG Le-Ping2,MIAO Xiong-Ying2,LI Long-Qing2 1 Department of Pathology,Third Xiangya Hospital,Changsha 410003,China,2Research Laboratory of Hepatobiliary Diseases,Second Xiangya Hospital,Central South University,Hunan 410011 |
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| Abstract: | AIM:To establish a model of pancreatic cancer in Sprague-Dawely(SD)rats,and to detect the expression of regulated proteins in cell junctions(Claudin-1,Occludin,E-cad,Snail)and their significances on the carcinogenesis in rats pancreas.METHODS:Ninety rats were randomly divided into A(n=40),B(n=40)and C(n=10)groups.For group A and B,dimethylbenzathracene,(DMBA)was directly implanted into the parenchyma of rat pancreas to establish pancreatic cancer model.The rats of group B were treated with trichostatin A(TS... |
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| Keywords: | pancreatic neoplasm sprague-Dawely rats animal model Claudins Occludin E-caherin Snail |
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