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| 超声辐照超声微泡介导siRNA 转染膀胱癌T24细胞的实验研究 | |
| 引用本文： | 宋希双,杨德勇,汪淑晶,车翔宇,王建伯,叶林,王丽娜. 超声辐照超声微泡介导siRNA 转染膀胱癌T24细胞的实验研究[J]. 中国医科大学学报, 2011, 40(9): 772-776 |
| 作者姓名： | 宋希双 杨德勇 汪淑晶 车翔宇 王建伯 叶林 王丽娜 |
| 作者单位： | 1. 大连医科大学附属第一医院泌尿外科,辽宁大连,116011 2. 大连医科大学生物化学教研室,辽宁大连,116011 3. 复旦大学附属肿瘤医院泌尿外科,上海,200032 |
| 基金项目： | 国家自然科学基金资助项目(30670544) |
| 摘 要： | 目的探讨超声辐照超声微泡介导siRNA 转染人膀胱癌T24 细胞的有效性。方法使用超声辐照超声微泡的方法介导FITC-siRNA转染人膀胱癌T24 细胞，通过荧光显微镜及流式细胞学方法观察siRNA的转染效率，使用MTT方法观察细胞活力。结果与各阴性对照组相比，超声辐照超声微泡的方法可以明显增加siRNA向T24 细胞中的转染效率。随着超声辐照强度的增加，siRNA的转染效率在一定范围内有所提高，同时也增加了对T24细胞毒性。与脂质体介导的siRNA体外转染相比，超声辐照超声微泡介导的siRNA 转染效率仍然较低。结论超声辐照超声微泡能够有效提高siRNA 转染膀胱癌细胞的效率，有可能为膀胱癌的基因靶向治疗提供新的转染手段。 |
| 关 键 词： | 膀胱癌 siRNA 超声微泡 转染 |
Delivery of siRNA to Bladder Cancer T24 Cells by Targeted Microbubble Destruction with Ultrasound |
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| SONG Xi-shuang , YANG De-yong , WANG Shu-jing , CHE Xiang-yu , WANG Jian-bo , YE Lin , WANG Li-na. Delivery of siRNA to Bladder Cancer T24 Cells by Targeted Microbubble Destruction with Ultrasound[J]. Journal of China Medical University, 2011, 40(9): 772-776 | |
| Authors: | SONG Xi-shuang YANG De-yong WANG Shu-jing CHE Xiang-yu WANG Jian-bo YE Lin WANG Li-na |
| Affiliation: | SONG Xi-shuang1,YANG De-yong1,WANG Shu-jing2,CHE Xiang-yu1,WANG Jian-bo1,YE Lin3,WANG Li-na1(1.First Affiliated Hospital,Dalian Medical University,Dalian 116011,China,2.Department of Biochemistry,3.Department of Urology,Fudan University Shanghai Cancer Center,Shanghai 200032,China) |
| Abstract: | Objective To evaluate the efficiency of delivering siRNA to T24 bladder cancer cells by microbubble destruction with ultrasound.Methods siRNA was labeled by FITC and transfected to T24 bladder cancer cells by targeted microbubble destruction with ultrasound.Transfection efficiency was observed by fluorescence microscope or flow cytometer.Cell viability was evaluated by MTT analysis.Results Compared to the negative controls,targeted microbubble destruction with ultrasound significantly increased the transfec... |
| Keywords: | bladder cancer siRNA microbubble destruction with ultrasound transfection |
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