生命早期前爪感觉和精细动作剥夺对大鼠空间学习记忆的影响

目的 研究生命早期前爪感觉和精细动作剥夺对大鼠海马依赖性空间参考学习记忆能力的影响。方法 新生SD大鼠随机分为前爪感觉和精细动作剥夺组（实验组,n=53）和假手术组（对照组，n=55)。取13日龄（PN13）实验组大鼠，运用显微外科技术建立幼鼠前爪感觉和精细动作剥夺模型。于PN25(PN21~31)、PN35(PN31~39)、PN45(PN41~50)和PN60(PN56~64)4个时间段，对两组大鼠分别进行旷场测试评估自发活动和探索能力；Morris 水迷宫测试评估空间参考学习记忆能力。结果 旷场测试中，两组大鼠于各时间段的自发活动各项指标比较差异无统计学意义 (P>0.05)。Morris水迷宫测试显示，在PN25和PN35时间段，对照组大鼠训练和测试阶段的成绩均显著高于实验组 (P<0.05)；在PN45时间段，训练阶段两组大鼠成绩比较差异无统计学意义(P>0.05)，测试阶段对照组大鼠成绩显著高于实验组 (P<0.05)。结论 生命早期前爪感觉和精细动作剥夺，对大鼠自发性粗大运动能力无明显影响，但可损伤其空间参考学习记忆能力。

Effects of forepaw sensorimotor deprivation in early life on spatial learning and memory in rats

Objective To explore the effects of forepaw sensorimotor deprivation in early life on hippocampus-dependent spatial reference learning and memory in rats. Methods Newborn SD rats were randomly assigned to experiment group (deprivation of forepaw sensorimotor function, n=53) and control group(n=55). Rats of postnatal day 13 (PN13) in experiment group were seleeted, and models of forepaw sensorimotor deprivation were established by microsurgical technique. Open field tests and Morris water maze tests were performed during the time periods of PN25(PN21-31), PN35 (PN31-39), PN45(PN41-50) and PN60(PN56-64) to evaluate the locomotor activity and spatial reference learning and memory, respectively. Results In open field tests, there was no significant difference in parameters of locomotor activity and exploratory behavior between the two groups (P>0.05). In Morris water maze tests, eontrol group performed significantly better than experiment group during training sessions and probe tests on PN25 and PN35 (P<0.05). While on PN45, although there was no significant difference between the two groups during training sessions, control group performed significantly better than experiment group during probe tests (P<0.05). Conclusion The deprivation of forepaw sensorimotor in early life has no signifieant effect on the locomotor activity and exploratory behavior of rats, but can impair the spatial reference learning and memory.