吡柔比星联合干扰素抑制大鼠膀胱癌生长的机制

 目的探讨吡柔比星联合干扰素琢鄄2b 膀胱灌注对大鼠膀胱肿瘤生长的抑制作用和机制。方法80 只雌性Wistar大鼠随机分为对照组、吡柔比星组、干扰素组和联合用药组，每组20 只。制作N鄄甲基亚硝基脲诱导大鼠原位膀胱癌模型，于第8周分别以生理盐水、吡柔比星、重组干扰素琢鄄2b 和吡柔比星+重组干扰素琢鄄2b 连续膀胱灌注，每周1 次，共6 次。第8 和14 周进行病理观察，第14 周测量膀胱质量，免疫组织化学法检测微血管密度（PMVD），流式细胞仪测凋亡指（PAI）。结果第8 周时，各组大鼠膀胱黏膜呈不典型增生和移行细胞癌玉级。第14 周时，可见菜花状、多发膀胱肿瘤并侵入肌层。吡柔比星组和联合用药组大膀胱质量小于对照组（P < 0.01），联合用药组大鼠膀胱质量小于吡柔比星组（P < 0.05），联合用药组MVD 小于干扰素组（P < 0.01），联合用药组大鼠的AI 高于其他3 组（P < 0.01），吡柔比星组和干扰素组的AI大于对照组（P 分别<0.01 和0.05）。结论吡柔比星联合干扰素琢鄄2b 膀胱灌注可抑制大鼠膀胱肿瘤生长，其机制与二者协同作用抑制肿瘤血管生长和诱导肿瘤细胞凋亡有关。

The Suppression Mechanism of the Growth of Bladder Cancer on Pirarubicin Combined with Interferon

Objective To observe the suppression mechanism of the growth of bladder cancer on pirarubicin combined with interferon.Methods 80 female Wistar rats were randomly divided into four groupscontrol group，pirarubicin group，interferon group and combination group. Establish N-nitrosourea Induction model of rat orthotopic bladder cancer，four groups were respectively received a total of six times（Pevery week）intravesical instillation of physiologic saline，pirarubicin，interferon（Pα-2b）and combination drugs from the eighth week，Patho-logical observation was done at the 8th week and the 14th week. The weights of bladders，microvessel densities（PMVD）and apoptotic index-es （PAI）were investigated. Results The mucosal of bladder was showed dysplasia and transitional cell I in both groups at the 8th week.Muscle layer of bladder could be seen cauliflower-like，multiple bladder tumors at the 14th week. The weight of bladder in pirarubicin groupand combination group were less than those of control group（P < 0.01），the latter was less than that of pirarubicin group （P < 0.05）. The MVD of combination group was less than that of interferon group （P < 0.01），and the AI of it was higher than that of other three groups（P< 0.01）. The AI of pirarubicin group and interferon group were higher than those of control group （P < 0.01， < 0.05）. Conclusion In-travesical instillation of pirarubicin and interferon could inhibit the growth of bladder tumour by restraining angiogenesis and accelerating apoptosis in rats.