MMP-2、TIMP-2的表达与骨巨细胞瘤复发的相关性

目的研究骨巨细胞瘤组织中基质金属蛋白酶-2(Matrix metalloproteinase-2)及其特异性抑制剂金属蛋白酶组织抑制因子-2(Tissue inhibitor of metalloproteinase-2)的表达与肿瘤血管形成和关系。方法用免疫组化SP法检测45例骨巨细胞瘤组织中MMP-2、TIMP-2的表达情况,以CD31标记检测微血管密度(MVD),计算肿瘤复发组和非复发组MMP-2/TIMP-2的比值,分析这些指标与骨巨细胞瘤预后的关系。结果骨巨细胞瘤组织有高水平的MMP-2、TIMP-2和CD31表达。复发组表达MMP-2、MMP-2/TIMP-2比值以及MVD均显著高于非复发组,但与组织学分级无关。MMP-2与MVD呈正的直线相关。结论MMP-2/TIMP-2比值升高与骨巨细胞瘤的复发以及肿瘤血管形成有关。

Relationship between expression of Matrix Metalloproteinase- 2, Tissue Inhibitors of Metalloproteinase- 2 and angiogenesis and recurrence of giant cell tumor of bone

Objective To investigate the relationship between expression of matrix metalloproteinase-2(MMP-2),tissue inhibitors of metalloproteinase-2(TIMP-2) and angiogenesis and recurrence of human giant cell tumor of bone. Methods Expression of MMP-2,TIMP-2,and CD31 in 45 patients with giant cell tumor of bone were detected by immunohistochemistry.The ratio of MMP-2/TIMP-2 was calculated and used as an indicator in comparison of recurrent rate of the tumor. Results Over-expression of MMP-2,TIMP-2,and micro vessel density(MVD) in giant cell tumors of bone was observed.The expression levels of MMP-2,ratio of MMP-2/TIMP-2 and MVD were significantly higher in recurrence group than non-recurrence group of the tumor.However,there was no relationship between expressions of MMP-2,TIMP-2,MVD and histological grading of the tumor.MMP-2 was positively correlated with MVD. Conclusion The recurrence of giant cell tumor of bone is correlated with high expression of MMP-2,MVD and high ratio of MMP-2/TIMP-2.