| Study on biocompatibility of PDLLA／HA／DBM with co-cultured human osteoblasts in vitro |
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| 引用本文： | 郭乔楠,赵建华,卢佳友.Study on biocompatibility of PDLLA／HA／DBM with co-cultured human osteoblasts in vitro[J].中国人民解放军军医大学学报,2003,18(5):277-282. |
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| 作者姓名： | 郭乔楠 赵建华 卢佳友 |
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| 作者单位： | [1]InstituteofPathology,SouthwestHospital,ThirdMilitaryMedicalUniversity,Chongqing400038,China [2]DepartmentofOrthopaedics,DapingHospital,ThirdMilitaryMedicalUniversity,Chongqing400042,China |
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| 摘 要： | Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDIZA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen I expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of bio-material and packed the granules of biomaterial. Conclusion: PDLLA/HA/DBM can form osteointerface early and have a good biocompability.
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| 关 键 词： | PDLLA／HA／DBM 骨组织工程 成骨细胞 生物相容性 骨软骨炎 |
Study on biocompatibility of PDLLA/HA/DBM with co-cultured human osteoblasts in vitro |
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| GUO Qiao-nan ZHAO Jian-hua LU Jia-you
Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing ,China.Study on biocompatibility of PDLLA/HA/DBM with co-cultured human osteoblasts in vitro[J].Journal of Medical Colleges of PLA(China),2003,18(5):277-282. |
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| Authors: | GUO Qiao-nan ZHAO Jian-hua LU Jia-you
Institute of Pathology Southwest Hospital Third Military Medical University Chongqing China |
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| Institution: | GUO Qiao-nan ZHAO Jian-hua LU Jia-you
Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China
Department of Orthopaedics,Daping Hospital,Third Military Medical University,Chongqing 400042,China |
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| Abstract: | Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix (PDLLA/HA/DBM), and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen Ⅰ expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of biomaterial and packed the granules of biomaterial. Conclusion: PDLLA/HA/DBM can form osteointerface early and have a good biocompability. |
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| Keywords: | biomaterial osteoblasts biocompatibility cell culture D L-polylactic/hydroxyapatite/decalcifying bone matrix(PDLLA/HA/DBM) |
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