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Rapid Identification of Bacteria and Candida Pathogens in Peritoneal Dialysis Effluent from Patients with Peritoneal Dialysis-Related Peritonitis by Use of Multilocus PCR Coupled with Electrospray Ionization Mass Spectrometry
Authors:Yu-Tzu Chang  Hsuan-Chen Wang  Ming-Cheng Wang  An-Bang Wu  Junne-Ming Sung  H. Sunny Sun  Ih-Jen Su  Wei-Chih Kan  Chih-Chiang Chien  Jyh-Chang Hwang  Hsien-Yi Wang  Chin-Chung Tseng  Chi-Jung Wu
Affiliation:aDepartment of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan;bNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan;cInstitute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan;dDepartment of Nephrology, Chi-Mei Medical Center, Tainan, Taiwan
Abstract:
PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.
Keywords:
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