Rapid Identification of Bacteria and Candida Pathogens in Peritoneal Dialysis Effluent from Patients with Peritoneal Dialysis-Related Peritonitis by Use of Multilocus PCR Coupled with Electrospray Ionization Mass Spectrometry |
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Authors: | Yu-Tzu Chang Hsuan-Chen Wang Ming-Cheng Wang An-Bang Wu Junne-Ming Sung H. Sunny Sun Ih-Jen Su Wei-Chih Kan Chih-Chiang Chien Jyh-Chang Hwang Hsien-Yi Wang Chin-Chung Tseng Chi-Jung Wu |
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Affiliation: | aDepartment of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan;bNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan;cInstitute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan;dDepartment of Nephrology, Chi-Mei Medical Center, Tainan, Taiwan |
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Abstract: | PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis. |
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