CD133、Glut-1在不同时期的血管瘤与血管畸形中的表达及意义

目的探讨CD133、葡萄糖转运蛋白-1（glucose transpoter1protein,Glut-1）及其所标记的前体细胞、胎盘绒毛微血管内皮细胞在血管瘤发生、发展及退化过程中的表达情况及其意义。方法采用SP免疫组织化学方法、Image-pro plus5.0图像分析技术检测人皮肤血管瘤增生期、退化期、先天性及后天性血管畸形中血管内皮细胞胞膜中的CD133和Glut-1的表达水平。结果不同时期的血管瘤、胎盘与先天性的血管畸形CD133均明显高于后天性的血管畸形,差异有统计学意义（P〈0.05）;增生期血管瘤和胎盘中的Glut-1的表达明显高于退化期血管瘤、血管畸形及后天性血管畸形,差异有统计学意义（P〈0.05）。结论CD133特异性标记的前体细胞---内皮祖细胞是先天性血管瘤及先天性血管畸形中大部分血管内皮细胞的来源。

Differential expression of CD133、 Glut-1 in tissues and endothelial cells derived from infantile hemangioma and vascular malformation

OBJECTIVE: To investigate the expression and significance of CD133, Glut-1 and precursor cell, placental microvessel endothelial cells in the occurrence, development and regression of infantile hemangiomas. METHODS: We examined the expression and significance of CD133, Glut-1 in the occurrence, development and regression of infantile hemangiomas and congenital vascular malformation postnatal vascular malformation using immunohistochemical technique. An image analysis system (Image-pro plus 5.0) was used to measure the average integrated optical density and the rate of positive area of CD133 and Glut-i in different stages of hemangiomas and in vascular malformation. RESULTS: The expression of CD133 was significantly higher in differential stages congential hemangioma, congential vascular malformation, placenta chorionic villi than postnatal vascular malformation (P < 0.05). About the expression of Glut-1, there was difference between proliferating hemangiomas, placenta and degenerating hemangiomas, vascular malformation (P < 0.05). CONCLUSION: The precursor cells marked CD133 is the source of endothelial cells derived from congential hemangiomas and congenital vascular malformation.