非小红细胞对血小板计数结果影响分析

目的 :探讨用血液分析仪作血常规检测时由非小红细胞引起的上限区域干扰 (upperregioninterference ,URI)对血小板计数 (BPC)结果的影响。方法 :用血液分析仪作血小板参数分析 ,对出现URI报警但又不是小红细胞干扰的标本作显微镜计数 ,把两法结果作配对t检验。结果 :仪器法结果为 (6 6± 17)× 10 9/L ,而显微镜计数法为 (98± 2 0 )× 10 9/L ,两法结果差异有显著性 (P <0 .0 0 1) ;URI报警时除小红细胞干扰外 ,还有其他诸多因素 ;大血小板、血小板凝集块、冷球蛋白、衰老破坏的红细胞碎片等 ,其中最常见的为大血小板。结论 :对URI报警但非小红细胞干扰的BPC结果 ,一定要进行人工显微镜目测计数复检 ,否则会得到不可靠的结果

Influence of nonmicrocyte on platelet count

Objective:To investigate the influence of up per region interference(URI) flag due to other than microcyte on blood platelet count in automated hematologic analyzer.Methods:Automated hematologic analyzer was used for platelet count if the URI flag was not due to the effect of microcyte,these samples were also c ounted under light microscope,and the results of these 2 methods were calculated for t test.Results:The results of these 2 methods were(66±17)×109/L and(98 ±2 0)×109/L,their difference was significant(P＜0.001).Their influential f actors were large platelets,platelet clumps,cryoglobulins and schistocytes besid es microcytes.Conclusions:The platelet count with URI flag not due to microcyte mu st be tested again under microscope,otherwise results are incredible.