| 半边旗中二萜类化合物5F对K562细胞MAPK活性、表达的影响 |
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| 引用本文： | 王京滨,梁念慈,莫丽儿. 半边旗中二萜类化合物5F对K562细胞MAPK活性、表达的影响[J]. 中国药理学通报, 2002, 18(3): 294-297 |
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| 作者姓名： | 王京滨 梁念慈 莫丽儿 |
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| 作者单位： | 1. 广东医学院生物化学与分子生物学研究所,湛江,524023
2. 广东医学院生物化学与分子生物学研究所化学教研室,湛江,524023 |
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| 基金项目： | 广东省科委重大科技攻关项目 No 962 2 0 0 7 0 0 2，国家自然科学基金资助课题 No 3 9870 90 0 |
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| 摘 要： | 目的 研究半边旗中二萜类化合物 5F(11α 羟基 15 氧 16 烯 对映贝壳杉烷 19酸 )对K5 6 2细胞MAPK活性、表达的影响。方法 5F作用K5 6 2细胞 2 4h后 ,以MBP为底物检测MAPK的活性、用蛋白印迹法测定MAPK的表达。结果 半边旗中二萜类化合物 5F作用于K5 6 2细胞使MAPK活性增强 ,表达增多 ,且有量效关系。结论 推测异常激活和表达的MAPK是化合物 5F抗肿瘤的机制之一
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| 关 键 词： | 半边旗 二萜类 丝裂原活化蛋白激酶 K562细胞 |
| 文章编号： | 1001-1978(2002)03-0294-04 |
| 修稿时间： | 2002-01-05 |
The effects of a diterpenoid compound 5F isolated from pteris semipinnata L on the activity and expression of mitogen activated protein kinase in K562 cells |
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| WANG Jing Bin,LIANG Nian Ci,MO Li Er. The effects of a diterpenoid compound 5F isolated from pteris semipinnata L on the activity and expression of mitogen activated protein kinase in K562 cells[J]. Chinese Pharmacological Bulletin, 2002, 18(3): 294-297 |
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| Authors: | WANG Jing Bin LIANG Nian Ci MO Li Er |
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| Affiliation: | WANG Jing Bin,LIANG Nian Ci,MO Li Er 1 |
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| Abstract: | AIM To study the effects of a diterpenoid compound 5F (ent 11α hydroxy 15 oxo kaur 16 en 19 oic acid) isolated from pteris semipinnata L on the the activity and expression of mitogen activated protein kinase of K562 cells. METHODS K562 cells were treated with compound 5F for 24 hours, then MAPK activity was determined using MBP as substrate ,Western blotting was used to detect the effect of compound 5F on the expression of MAPK. RESULTS The activity and expression of MAPK increased after the treatment of 5F. CONCLUSION One mechanism of the anti tumor effect of compound 5F is through abnormal activation and abnormal expression of MAPK. |
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| Keywords: | pteris semipinnata L diterpenoid mitogen activated protein kinase K562 cells |
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