| 银杏叶提取物对大鼠脑缺血再灌注后Bcl-2、Bax蛋白和Caspase-3表达的影响 |
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| 引用本文： | 李俊玲,牟凯,赵冬梅. 银杏叶提取物对大鼠脑缺血再灌注后Bcl-2、Bax蛋白和Caspase-3表达的影响[J]. 滨州医学院学报, 2011, 34(3): 187-188,191 |
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| 作者姓名： | 李俊玲 牟凯 赵冬梅 |
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| 作者单位： | 桓台县人民医院,256400;淄博市第三人民医院;滨州医学院人体解剖学教研室 |
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| 摘 要： | 目的探讨银杏叶提取物（ginkgo biloba extract,GBE）对脑缺血再灌注后Bcl-2、Bax蛋白和半胱氨酸蛋白酶-3（Caspase-3）表达的影响。方法将SD大鼠随机分为假手术组（A组）、缺血再灌注组（B组）和GBE治疗组（C组）,建立大鼠大脑中动脉缺血1.5 h再灌注24 h的模型;C组于缺血前30 min及缺血再灌注后1 h腹腔内注射银杏叶提取物;采用免疫组化SP法检测脑缺血再灌注后Bcl-2、Bax蛋白的表达,采用四肽酶底物法检测Caspase-3活性水平。结果 GBE治疗组大鼠Bcl-2阳性细胞表达数目均高于缺血再灌注组,Bax少于缺血再灌注组（P〈0.05）;假手术组未检测到Caspase-3活性升高,缺血再灌注组Caspase-3活性明显高于GBE组,有统计学意义（P〈0.05）。结论 GBE可通过上调Bcl-2蛋白表达而减少Caspase-3活性水平,下调Bax蛋白表达,来降低脑缺血再灌注后大鼠神经细胞凋亡。
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| 关 键 词： | 银杏叶提取物 Bcl-2蛋白 Bax蛋白 半胱氨酸蛋白酶-3 缺血再灌注 |
Effects of ginkgo biloba extract on the expression of Bcl-2,Bax proteins and activities of Caspase-3 following ischemic reperfusion injury in rats |
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| LI Junling,MU Kai,ZHAO Dongmei. Effects of ginkgo biloba extract on the expression of Bcl-2,Bax proteins and activities of Caspase-3 following ischemic reperfusion injury in rats[J]. Journal of Binzhou Medical College, 2011, 34(3): 187-188,191 |
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| Authors: | LI Junling MU Kai ZHAO Dongmei |
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| Affiliation: | LI Junling1 MU Kai2 ZHAO Dongmei31 People's Hospital of Huantai 256400,2 third People's Hospital of Zibo,3 Department of Human Anatomy,Binzhou Medical University |
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| Abstract: | Objective To explore the influence of ginkgo biloba extract(GBE)on the expression of Bcl-2,Bax proteins and activities of Caspase-3 following ischemic reperfusion injury in rats.Methods SD rats were randomly divided into control group(group A),ischemic reperfusion group(group B),GBE group(group C).The model of cerebral artery ischemic and reperfusion was setted up.GBE of 40 mg/kg were given intraperitoneally to rats in group C 30 min before focal cerebral ischemia operation and 1 h after ischemic reperfusio... |
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| Keywords: | ginkgo biloba extract(GBE) Bcl-2 protein Bax protein Caspase-3 ischemic reperfusion |
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