缝裂层孔菌对荷瘤小鼠放、化疗的减毒增效作用

目的：通过缝裂层孔菌（PL）与放、化疗联合应用于荷瘤小鼠，探讨PL对放、化疗的减毒增效作用。方法：小鼠右前肢腋下荷瘤细胞胃癌（MFC细胞株），随机分13组，即空白对照组，及单纯CTX高、中和低3个剂量组（20、10和5 mg•kg^-1），以及PL高、中和低3个剂量（2.0、1.0和0.5 g•kg^-1）分别与CTX 3个剂量联合给药组。空白对照组每日灌胃给予蒸馏水0.01 mL•g^-1；CTX为隔日腹腔注射给药；PL为每日灌胃给药，连续给药10 d。停药日处死小鼠，应用重量法观察各组荷瘤（胃癌）小鼠体重和瘤重的变化。小鼠右后肢胫骨外侧皮下荷瘤细胞肝癌（HepA细胞株），随机分5组，即空白对照组、单纯X线放疗组、PL 3个剂量分别与放疗联合治疗组。空白对照组每日灌胃给予蒸馏水0.01 mL•g^-1；PL为每日灌胃给药，连续14 d；X线照射于PL给药第5天开始，每次照射0.5 Gy，每日1次，共5次。PL停药后次日处死小鼠，应用重量法观察各组荷瘤（肝癌）小鼠体重和瘤重的变化。结果：单纯CTX 3个剂量和分别与PL 3个剂量联合给药各组荷瘤（胃癌）小鼠瘤重较空白对照组均明显降低（P<0.01）； CTX和PL联合给药各组荷瘤（胃癌）小鼠瘤重较单纯同剂量CTX组均明显降低（P<0.05或P<0.01)。与空白对照组比较，单纯应用CTX 3个剂量组荷瘤（胃癌）小鼠体重均明显降低（P<0.05或P<0.01)，CTX和PL联合给药各组荷瘤（胃癌）小鼠体重均无明显变化。单纯X线放疗和分别与PL高、中和低3个剂量联合应用各组荷瘤（肝癌）小鼠瘤重较空白对照组均明显降低（P<0.01），不同剂量PL和X线联合应用各组荷瘤（肝癌）小鼠瘤重较单纯X线放疗组均显著降低（P<0.05或P<0.01)。与空白对照组比较，单纯X线放疗组荷瘤（肝癌）小鼠体重明显降低（P<0.05），不同剂量PL和X线联合应用各组荷瘤（肝癌）小鼠体重均无明显变化。结论：PL与放疗（CTX）、化疗（X线）联合应用，在治疗荷瘤（胃癌、肝癌）小鼠中有协同作用，并且PL对放、化疗有减毒增效作用。

Efficacy enhancing and side-effects reducing of Phellinus linteus on radiotherapy or chemotherapy in mice bearing tumor

Objective To study efficacy enhancing and side-effects reducing of Phellinus linteus(PL) on radiotherapy or chemotherapy in mice bearing tumor.Methods MFC were implanted subcutaneously to the right anterior limb of mice and the mice were randomly divided into thirteen groups: control,CTX groups(20,10 and 5 mg·kg-1)and groups of PL(2.0,1.0 and 0.5 g·kg-1)with CTX(20,10 and 5 mg·kg-1),respectively.Mice in control group were administered intragastricly at a dose 0.01 mL·g-1 for 10 d;PL were administered intragastricly for 10 d;CTX were administered parenterally every other day for 10 d.The body weight and the tumor weight of mice were measured.HepA were implanted subcutaneously to the lateral side of tibia of the right posterior limb of mice and the mice were randomly divided into five groups: control,radiotherapy group and groups of PL(2.0,1.0 and 0.5 g·kg-1)with radiotherapy,respectively.Mice in control group were administered intragastricly with distilled water at a dose of 0.01 mL·g-1 for 14 d;PL was administered intragastricly for 14 d;after PL was administered for 5 d,X-rays were utilized at a dose of 0.5 Gy one time everday for 5 d.The body weight and the tumor weight of mice were measured.Results Compared with control group, the tumor weights decreased significantly in CTX groups and groups of PL with CTX (P<0.01).Compared with the groups of the equal-dose CTX,the tumor weights in groups of PL with CTX respectively (P<0.05 or P<0.01).Compared with control group,the body weights decreased in CTX groups(P<0.05 or P<0.01),but the body weights in groups of PL with CTX didn't change remarkably.Compared with control group,the tumor weights decreased in radiotherapy group and groups of PL with radiotherapy (P<0.01).Compared with radiotherapy group,the tumor weights decreased in groups of PL with radiotherapy (P<0.05 or P<0.01).Compared with control group,the body weight decreased in radiotherapy group(P<0.05),but the body weights in groups of PL with radiotherapy didn't change remarkably.Conclusion PL is in coordination with radiotherapy or chemotherapy on anti-tumor,and PL can enhance efficacy and reduce side-effects of radiotherapy or chemotherapy.