Strategies for expressing human antibody repertoires in transgenic mice |
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| Affiliation: | 1. The Dept of Development and Genetics, Laboratory of Developmental Immunology, The Babraham Institute, Babraham, Cambridge, UK CB2 4AT;2. The Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, UK CB2 2QH;1. Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, 615 Charles E. Young Drive, Los Angeles, CA 90095, United States;2. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, 615 Charles E. Young Drive, CA 90095, United States;1. Institutes of Biology and Medical Sciences, Soochow University, Suzhou, Jiangsu Province 215000, China;2. MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Medical College of Soochow University, Suzhou, Jiangsu Province 215000, China;3. Department of Respiratory Medicine, Children''s Hospital of Soochow University, Suzhou, Jiangsu Province 215000, China;1. Department of Gastroenterology, Shanghai Tenth People''s Hospital, Tongji University, Shanghai, China;2. Department of Central Laboratory, Shanghai Tenth People''s Hospital, Tongji University, Shanghai, China;3. Institute of Intervention Vessel, Tongji University School of Medicine, Shanghai, China;4. Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Tex;1. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA;2. Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;3. Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA;4. Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA;5. Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA |
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| Abstract: | Repertoires of human antibodies can be created in transgenic mice carrying human immunoglobulin-gene loci in germline configuration. These ‘transloci’, introduced either as miniloci or as almost locus-sized regions, undergo rearrangement and hypermutation in mouse lymphoid tissue. Here, Marianne Brüggemann and Michael Neuberger review the use of such mice for raising antigen-specific human monoclonal antibodies, as well as their exploitation for studying regulatory aspects of antibody repertoire formation. |
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